Protoporphyrin IX distribution in rat brain following the administration of 5-aminolevulinic acid and its hexylester

Rowett nude rats were injected intra-cranially with varying concentrations of 5-aminolevulinic acid (10, 20, 99, 199, 398 mM) and its hexylester (6.6, 13, 66, 132, 265 mM). Toxicity effects of these compounds were examined based on animal behavior and respiratory distress. It was determined that the maximum tolerable dose for intra-cranial injection is 99 mM 5-Aminolevulinic acid and 66 mM h-ALA; Human glioma spheroids were grown in vitro. After incubation, spheroids were sized (250--350 mum) and injected into the rat brain (10 spheroids per injection). Following the induction and development of glioblastoma multiforme tumors in vivo, Rowett nude rats were injected with the maximum tolerable ALA or h-ALA dose. Animals were sacrificed four hours post-injection and intact brains were removed. Fluorescence microscopy was performed to quantify PpIX production. Results indicate that PpIX production in tumor tissue is greater following 5-aminolevulinic acid administration than h-ALA. However, the tumor-to-normal tissue uptake ratio is superior following injection of h-ALA (7.6 +/- 2.0:1) compared to 5-aminolevulinic acid (2.4 +/- 1.1:1).

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Health Physics and Diagnostic Sciences

Grantor dc:publisher

University of Nevada, Las Vegas

Year

2001

Author dc:creator

• Friesen, Scott Alan

Contributors dc:contributor

• Steen J. Madsen

Statement dc:rights

• IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/

Language dc:language

English

Identifier

https://oasis.library.unlv.edu/rtds/1360

OAI identifier oai:identifier

oai:oasis.library.unlv.edu:rtds-2359