{"id":{"repo_id":"unlv","oai_identifier":"oai:oasis.library.unlv.edu:rtds-2359"},"canonical_url":"https://search.dev.ndltd.org/etd/unlv/oai:oasis.library.unlv.edu:rtds-2359","repository":{"repo_id":"unlv","name":"University of Nevada - Las Vegas","base_url":"https://oasis.library.unlv.edu/do/oai/"},"display":{"title":"Protoporphyrin IX distribution in rat brain following the administration of 5-aminolevulinic acid and its hexylester","abstract":"Rowett nude rats were injected intra-cranially with varying concentrations of 5-aminolevulinic acid (10, 20, 99, 199, 398 mM) and its hexylester (6.6, 13, 66, 132, 265 mM). Toxicity effects of these compounds were examined based on animal behavior and respiratory distress. It was determined that the maximum tolerable dose for intra-cranial injection is 99 mM 5-Aminolevulinic acid and 66 mM h-ALA; Human glioma spheroids were grown in vitro. After incubation, spheroids were sized (250--350 mum) and injected into the rat brain (10 spheroids per injection). Following the induction and development of glioblastoma multiforme tumors in vivo, Rowett nude rats were injected with the maximum tolerable ALA or h-ALA dose. Animals were sacrificed four hours post-injection and intact brains were removed. Fluorescence microscopy was performed to quantify PpIX production. Results indicate that PpIX production in tumor tissue is greater following 5-aminolevulinic acid administration than h-ALA. However, the tumor-to-normal tissue uptake ratio is superior following injection of h-ALA (7.6 +/- 2.0:1) compared to 5-aminolevulinic acid (2.4 +/- 1.1:1).","abstract_html":"Rowett nude rats were injected intra-cranially with varying concentrations of 5-aminolevulinic acid (10, 20, 99, 199, 398 mM) and its hexylester (6.6, 13, 66, 132, 265 mM). Toxicity effects of these compounds were examined based on animal behavior and respiratory distress. It was determined that the maximum tolerable dose for intra-cranial injection is 99 mM 5-Aminolevulinic acid and 66 mM h-ALA; Human glioma spheroids were grown in vitro. After incubation, spheroids were sized (250--350 mum) and injected into the rat brain (10 spheroids per injection). Following the induction and development of glioblastoma multiforme tumors in vivo, Rowett nude rats were injected with the maximum tolerable ALA or h-ALA dose. Animals were sacrificed four hours post-injection and intact brains were removed. Fluorescence microscopy was performed to quantify PpIX production. Results indicate that PpIX production in tumor tissue is greater following 5-aminolevulinic acid administration than h-ALA. However, the tumor-to-normal tissue uptake ratio is superior following injection of h-ALA (7.6 +/- 2.0:1) compared to 5-aminolevulinic acid (2.4 +/- 1.1:1).","abstract_has_math":false,"creators":["Friesen, Scott Alan"],"institution":"University of Nevada, Las Vegas","degree_name":"Master of Science (MS)","degree_level":"Thesis","degree_discipline":"Health Physics and Diagnostic Sciences","degree_department":null,"school":null,"contributors":["Steen J. Madsen"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2001,"date_issued":"2001-01-01T08:00:00Z","date_published":"2001-01-01T08:00:00Z","updated_at":"2026-07-24T05:25:26Z","subjects":[],"languages":["English"],"rights":["IN COPYRIGHT. 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For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["10.25669/ycei-82me","https://oasis.library.unlv.edu/rtds/1360","https://oasis.library.unlv.edu/context/rtds/article/2359/viewcontent/uc.pdf"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Rowett nude rats were injected intra-cranially with varying concentrations of 5-aminolevulinic acid (10, 20, 99, 199, 398 mM) and its hexylester (6.6, 13, 66, 132, 265 mM). Toxicity effects of these compounds were examined based on animal behavior and respiratory distress. It was determined that the maximum tolerable dose for intra-cranial injection is 99 mM 5-Aminolevulinic acid and 66 mM h-ALA; Human glioma spheroids were grown in vitro. After incubation, spheroids were sized (250--350 mum) and injected into the rat brain (10 spheroids per injection). Following the induction and development of glioblastoma multiforme tumors in vivo, Rowett nude rats were injected with the maximum tolerable ALA or h-ALA dose. Animals were sacrificed four hours post-injection and intact brains were removed. Fluorescence microscopy was performed to quantify PpIX production. Results indicate that PpIX production in tumor tissue is greater following 5-aminolevulinic acid administration than h-ALA. However, the tumor-to-normal tissue uptake ratio is superior following injection of h-ALA (7.6 +/- 2.0:1) compared to 5-aminolevulinic acid (2.4 +/- 1.1:1)."]},{"key":"dc:format","label":"Dc Format","values":["pdf"]},{"key":"dc:title","label":"Title","values":["Protoporphyrin IX distribution in rat brain following the administration of 5-aminolevulinic acid and its hexylester"]}]}],"canonical_facts":{"dc:contributor":["Steen J. Madsen"],"dc:creator":["Friesen, Scott Alan"],"dc:description.abstract":["Rowett nude rats were injected intra-cranially with varying concentrations of 5-aminolevulinic acid (10, 20, 99, 199, 398 mM) and its hexylester (6.6, 13, 66, 132, 265 mM). Toxicity effects of these compounds were examined based on animal behavior and respiratory distress. It was determined that the maximum tolerable dose for intra-cranial injection is 99 mM 5-Aminolevulinic acid and 66 mM h-ALA; Human glioma spheroids were grown in vitro. After incubation, spheroids were sized (250--350 mum) and injected into the rat brain (10 spheroids per injection). Following the induction and development of glioblastoma multiforme tumors in vivo, Rowett nude rats were injected with the maximum tolerable ALA or h-ALA dose. Animals were sacrificed four hours post-injection and intact brains were removed. Fluorescence microscopy was performed to quantify PpIX production. Results indicate that PpIX production in tumor tissue is greater following 5-aminolevulinic acid administration than h-ALA. However, the tumor-to-normal tissue uptake ratio is superior following injection of h-ALA (7.6 +/- 2.0:1) compared to 5-aminolevulinic acid (2.4 +/- 1.1:1)."],"dc:format":["pdf"],"dc:identifier":["10.25669/ycei-82me","https://oasis.library.unlv.edu/rtds/1360","https://oasis.library.unlv.edu/context/rtds/article/2359/viewcontent/uc.pdf"],"dc:language":["English"],"dc:publisher":["University of Nevada, Las Vegas"],"dc:rights":["IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/"],"dc:title":["Protoporphyrin IX distribution in rat brain following the administration of 5-aminolevulinic acid and its hexylester"],"dc:type":["Text"],"thesis:degree_discipline":["Health Physics and Diagnostic Sciences"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T05:25:26Z"}