Evaluation of selected aliphatic acids and polytetrahydrofurans for use as calibration agents for thermospray Hplc/Ms

A method of tuning and calibration for a thermospray ion source on a benchtop quadrupole mass spectrometer using trifluoroacetic acid, pentafluoropropionic acid, heptafluorobutyric acid, polytetrahydrofuran-250, and polytetrahydrofuran-650 was proposed and evaluated. A 0.5% solution of the aliphatic acids showed good mass range coverage (163-641) in the negative mode, and no substantial memory effects. This mixture was evaluated over 45 days for fluctuations in the ion intensities and ratios. It remained stable within the expected thermospray reproducibility range, although the volatility of the trifluoroacetic acid made daily preparation of standard mixtures necessary. A similar solution of the polytetrahydrofurans was evaluated in both the positive and the negative ionization mode. While several high mass ions were identified in the resulting spectra, these compounds lacked the reproducibility and predictability required, and were dropped from further evaluation.

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Chemistry

Grantor dc:publisher

University of Nevada, Las Vegas

Year

1993

Author dc:creator

• Dombrowski, Tonya Rose

Contributors dc:contributor

• Vernon Hodge

Statement dc:rights

• IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/

Language dc:language

English

Identifier

https://oasis.library.unlv.edu/rtds/285

OAI identifier oai:identifier

oai:oasis.library.unlv.edu:rtds-1284