2,3-butanediol production from cellobiose by engineered Saccharomyces cerevisiae

Production of renewable chemicals from cellulosic biomass is a critical step towards energy sustainability and reduced greenhouse gas emissions. Microbial cells have been engineered for producing fuels and chemicals from cellulosic sugars. Among these chemicals, 2,3-butanediol (2,3-BDO) is a compound of interest due to its diverse applications. While microbial production of 2,3-BDO with high yields and productivities has been reported, there are concerns with the use of potential pathogenic bacteria and inefficient utilization of cellulosic sugars. To address these problems, we engineered Saccharomyces cerevisiae to produce 2,3-BDO, especially from cellobiose which is a prevalent sugar in cellulosic hydrolyzates. Specifically, we overexpressed alsS and alsD from Bacillus subtilis to convert pyruvate to 2,3-BDO via α-acetolactate and acetoin in engineered S. cerevisiae capable of fermenting cellobiose directly. Under oxygen-limited conditions, the resulting strain was able to produce 2,3-BDO. Still, the majority of carbon flux in the strain went to ethanol, resulting in significant amounts of ethanol production. To enhance pyruvate flux to 2,3-BDO through elimination of the pyruvate decarboxylation (PDC) reaction, we employed a deletion mutant of both PDC1 and PDC5 for producing 2,3-BDO from cellobiose. The subsequent strain was able to produce only 2,3-BDO without ethanol production from cellobiose under oxygen-limited conditions. These results suggest the possibility of producing 2,3-BDO safely and sustainably from cellulosic hydrolyzates.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Food Science & Human Nutrition

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2016

Author dc:creator

• Nan, Hong

Contributors dc:contributor

• Jin, Yong-Su

• pyruvate decarboxylase deletion
• cellobiose
• 2,3-butanediol
• Saccharomyces cerevisiae

Statement dc:rights

• Copyright 2013 Hong Nan

Handle dc:identifier

http://hdl.handle.net/2142/90064

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/90064