{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/90064"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/90064","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"2,3-butanediol production from cellobiose by engineered Saccharomyces cerevisiae","abstract":"Production of renewable chemicals from cellulosic biomass is a critical step towards energy sustainability and reduced greenhouse gas emissions. Microbial cells have been engineered for producing fuels and chemicals from cellulosic sugars. Among these chemicals, 2,3-butanediol (2,3-BDO) is a compound of interest due to its diverse applications. While microbial production of 2,3-BDO with high yields and productivities has been reported, there are concerns with the use of potential pathogenic bacteria and inefficient utilization of cellulosic sugars. To address these problems, we engineered Saccharomyces cerevisiae to produce 2,3-BDO, especially from cellobiose which is a prevalent sugar in cellulosic hydrolyzates. Specifically, we overexpressed alsS and alsD from Bacillus subtilis to convert pyruvate to 2,3-BDO via α-acetolactate and acetoin in engineered S. cerevisiae capable of fermenting cellobiose directly. Under oxygen-limited conditions, the resulting strain was able to produce 2,3-BDO. Still, the majority of carbon flux in the strain went to ethanol, resulting in significant amounts of ethanol production. To enhance pyruvate flux to 2,3-BDO through elimination of the pyruvate decarboxylation (PDC) reaction, we employed a deletion mutant of both PDC1 and PDC5 for producing 2,3-BDO from cellobiose. The subsequent strain was able to produce only 2,3-BDO without ethanol production from cellobiose under oxygen-limited conditions. These results suggest the possibility of producing 2,3-BDO safely and sustainably from cellulosic hydrolyzates.","abstract_html":"Production of renewable chemicals from cellulosic biomass is a critical step towards energy sustainability and reduced greenhouse gas emissions. Microbial cells have been engineered for producing fuels and chemicals from cellulosic sugars. Among these chemicals, 2,3-butanediol (2,3-BDO) is a compound of interest due to its diverse applications. While microbial production of 2,3-BDO with high yields and productivities has been reported, there are concerns with the use of potential pathogenic bacteria and inefficient utilization of cellulosic sugars. To address these problems, we engineered Saccharomyces cerevisiae to produce 2,3-BDO, especially from cellobiose which is a prevalent sugar in cellulosic hydrolyzates. Specifically, we overexpressed alsS and alsD from Bacillus subtilis to convert pyruvate to 2,3-BDO via α-acetolactate and acetoin in engineered S. cerevisiae capable of fermenting cellobiose directly. Under oxygen-limited conditions, the resulting strain was able to produce 2,3-BDO. Still, the majority of carbon flux in the strain went to ethanol, resulting in significant amounts of ethanol production. To enhance pyruvate flux to 2,3-BDO through elimination of the pyruvate decarboxylation (PDC) reaction, we employed a deletion mutant of both PDC1 and PDC5 for producing 2,3-BDO from cellobiose. The subsequent strain was able to produce only 2,3-BDO without ethanol production from cellobiose under oxygen-limited conditions. These results suggest the possibility of producing 2,3-BDO safely and sustainably from cellulosic hydrolyzates.","abstract_has_math":false,"creators":["Nan, Hong"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"M.S.","degree_level":"Thesis","degree_discipline":"Food Science & Human Nutrition","degree_department":null,"school":null,"contributors":["Jin, Yong-Su"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2016,"date_issued":"2016-05-04T21:49:05Z","date_published":"2016-05-04T21:49:05Z","updated_at":"2026-07-22T22:26:32Z","subjects":["pyruvate decarboxylase deletion","cellobiose","2,3-butanediol","Saccharomyces cerevisiae"],"languages":[],"rights":["Copyright 2013 Hong Nan"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"http://hdl.handle.net/2142/90064","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Jin, Yong-Su"]},{"key":"dc:creator","label":"Author","values":["Nan, Hong"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2016-05-04T21:49:05Z","2013-07-19","2013-08"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science & Human Nutrition"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["M.S."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["pyruvate decarboxylase deletion","cellobiose","2,3-butanediol","Saccharomyces cerevisiae"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:rights","label":"Dc Rights","values":["Copyright 2013 Hong Nan"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/90064"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Production of renewable chemicals from cellulosic biomass is a critical step towards energy sustainability and reduced greenhouse gas emissions. Microbial cells have been engineered for producing fuels and chemicals from cellulosic sugars. Among these chemicals, 2,3-butanediol (2,3-BDO) is a compound of interest due to its diverse applications. While microbial production of 2,3-BDO with high yields and productivities has been reported, there are concerns with the use of potential pathogenic bacteria and inefficient utilization of cellulosic sugars. To address these problems, we engineered Saccharomyces cerevisiae to produce 2,3-BDO, especially from cellobiose which is a prevalent sugar in cellulosic hydrolyzates. Specifically, we overexpressed alsS and alsD from Bacillus subtilis to convert pyruvate to 2,3-BDO via α-acetolactate and acetoin in engineered S. cerevisiae capable of fermenting cellobiose directly. Under oxygen-limited conditions, the resulting strain was able to produce 2,3-BDO. Still, the majority of carbon flux in the strain went to ethanol, resulting in significant amounts of ethanol production. To enhance pyruvate flux to 2,3-BDO through elimination of the pyruvate decarboxylation (PDC) reaction, we employed a deletion mutant of both PDC1 and PDC5 for producing 2,3-BDO from cellobiose. The subsequent strain was able to produce only 2,3-BDO without ethanol production from cellobiose under oxygen-limited conditions. These results suggest the possibility of producing 2,3-BDO safely and sustainably from cellulosic hydrolyzates.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2016-05-04 without embargo terms","The student, Hong Nan, accepted the attached license on 2013-07-18 at 13:22.","The student, Hong Nan, submitted this Thesis for approval on 2013-07-18 at 17:08.","This Thesis was approved for publication on 2013-07-19 at 12:18.","DSpace SAF Submission Ingestion Package generated from Vireo submission #5689 on 2016-05-04 at 16:34:25","Made available in DSpace on 2016-05-04T21:49:05Z (GMT). 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While microbial production of 2,3-BDO with high yields and productivities has been reported, there are concerns with the use of potential pathogenic bacteria and inefficient utilization of cellulosic sugars. To address these problems, we engineered Saccharomyces cerevisiae to produce 2,3-BDO, especially from cellobiose which is a prevalent sugar in cellulosic hydrolyzates. Specifically, we overexpressed alsS and alsD from Bacillus subtilis to convert pyruvate to 2,3-BDO via α-acetolactate and acetoin in engineered S. cerevisiae capable of fermenting cellobiose directly. Under oxygen-limited conditions, the resulting strain was able to produce 2,3-BDO. Still, the majority of carbon flux in the strain went to ethanol, resulting in significant amounts of ethanol production. To enhance pyruvate flux to 2,3-BDO through elimination of the pyruvate decarboxylation (PDC) reaction, we employed a deletion mutant of both PDC1 and PDC5 for producing 2,3-BDO from cellobiose. The subsequent strain was able to produce only 2,3-BDO without ethanol production from cellobiose under oxygen-limited conditions. These results suggest the possibility of producing 2,3-BDO safely and sustainably from cellulosic hydrolyzates.","Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2016-05-04 without embargo terms","The student, Hong Nan, accepted the attached license on 2013-07-18 at 13:22.","The student, Hong Nan, submitted this Thesis for approval on 2013-07-18 at 17:08.","This Thesis was approved for publication on 2013-07-19 at 12:18.","DSpace SAF Submission Ingestion Package generated from Vireo submission #5689 on 2016-05-04 at 16:34:25","Made available in DSpace on 2016-05-04T21:49:05Z (GMT). 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