Investigating lipid corona formation onto polystyrene nanoparticles through fluorescence correlation spectroscopy

When a nanoparticle enters a biological environment, molecules are known to adsorb on the surface forming a corona. Systematically studying the formation of a corona is important to develop knowledge as to how a given nanomaterial will transform once entering a biological environment. A common interface met by a nanomaterial in the human body is the cell membrane, which is composed of phospholipid bilayer. Fluorescence correlation spectroscopy (FCS) is a promising tool that can be used to probe nanoparticle-cell interactions. FCS experiments focusing on exposure of lipid vesicles to different polystyrene nanoparticles indicate possible formation of a lipid corona. Further study varying the nanoparticle charge and lipid vesicle fluidity can help elucidate the mechanism of lipid corona formation. Such work can provide insight into understanding the complex nature of the nano-bio interface.

Name thesis:degree_name

M.S.

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2016

Author dc:creator

• Jacob, Lisa

Contributors dc:contributor

• Murphy, Catherine J.

• nano-bio interactions
• fluorescence correlation spectroscopy
• lipid corona

Statement dc:rights

• Copyright 2015 Lisa Jacob

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/89156

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/89156