Design, characterization, and reinforcement of mineralized collagen-glycosaminoglycan scaffolds for orthopedic wound repair

The treatment of craniofacial orthopedic defects remains a challenge due to the large size, complex geometry, and load bearing aspects that often accompany such defects. The typical treatment or correction of craniofacial defects employed during surgical intervention of such injuries often necessitates the use of either autologous or allogenic bone grafts for orthopedic implants as the gold standard. However, this standard of treatment has been plagued with concerns over the size, geometry, donor site morbidity, disease transmission, and availability. To address these concerns, the development of an alternative orthopedic implant biomaterial capable of: (1) flexible design for meeting bone, defect, and patient specific treatment criteria; (2) guiding and supporting cellular specific repair specific to orthopedic defects; (3) demonstrate mechanical competence for surgical implantation and subsequent loading during function. This work outlines the development of a collagen-glycosaminoglycan platform that satisfies these requirements for the treatment of craniofacial defects. Chapter 2 details the fabrication and characterization of the collagen-glycosaminoglycan scaffold biochemical and biophysical properties important for the bioactivity and interaction with target cellular populations. The chapter 3 evaluates the in vitro cellular response and subsequent remodeling of a relevant cell population, mesenchymal stem cells, within the collagen-glycosaminoglycan scaffold. Chapter 4 addresses the necessary mechanical reinforcement and subsequent characterization of a multi-scale polycaprolactone-collagen-glycosaminoglycan composite biomaterial for in vivo implantation and culture. In chapter 5 the polycaprolactone-collagen-glycosaminoglycan composite was then implanted in a subcritical mandibular ramus defect to affirm the composites in vivo efficacy. Finally, chapter 6 evaluated possible alterations in the collagen-glycosaminoglycan scaffold design for enhancing cellular behavior, while chapter 7 expanded upon the multi-compartment collagen-glycosaminoglycan scaffold design for the treatment of craniofacial defects and associated entheses.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Materials Science & Engr

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2016

Author dc:creator

• Weisgerber, Daniel W

Contributors dc:contributor

• Harley, Brendan
• Braun, Paul
• Cheng, Jianjun
• Leal, Cecilia

• collagen scaffold
• biomaterial
• craniofacial repair
• bone

Statement dc:rights

• Copyright 2015 Daniel W. Weisgerber

Language dc:language

en

Handle dc:identifier

http://hdl.handle.net/2142/89143

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/89143