Osmoregulation in Trypanosoma Cruzi: The Role of the Acidocalcisome

Electron microscopic observations revealed a contractile vacuole coupled to acidocalcisomes in all three life cycle stages of the parasite. An aquaporin homologue localized to the acidocalcisomes and contractile vacuole. In epimastigotes expressing a GFP-aquaporin fusion protein, acidocalcisomes trafficked toward the contractile vacuole during hyposmotic stress in a cAMP- and microtubule-dependent fashion. This phenomenon was functionally significant, since aquaporin and microtubule inhibitors reduced the rate of volume recovery. Isolated contractile vacuoles were enriched in alkaline phosphatase, aquaporin, and some acidocalcisomal markers.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Veterinary Pathobiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Rohloff, Peter John

Contributors dc:contributor

• Roberto Docampo

• Health Sciences, Pathology

Language dc:language

eng

Identifier

(MiAaPQ)AAI3101957

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/87623