{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/87623"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/87623","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Osmoregulation in Trypanosoma Cruzi: The Role of the Acidocalcisome","abstract":"Electron microscopic observations revealed a contractile vacuole coupled to acidocalcisomes in all three life cycle stages of the parasite. An aquaporin homologue localized to the acidocalcisomes and contractile vacuole. In epimastigotes expressing a GFP-aquaporin fusion protein, acidocalcisomes trafficked toward the contractile vacuole during hyposmotic stress in a cAMP- and microtubule-dependent fashion. This phenomenon was functionally significant, since aquaporin and microtubule inhibitors reduced the rate of volume recovery. Isolated contractile vacuoles were enriched in alkaline phosphatase, aquaporin, and some acidocalcisomal markers.","abstract_html":"Electron microscopic observations revealed a contractile vacuole coupled to acidocalcisomes in all three life cycle stages of the parasite. An aquaporin homologue localized to the acidocalcisomes and contractile vacuole. In epimastigotes expressing a GFP-aquaporin fusion protein, acidocalcisomes trafficked toward the contractile vacuole during hyposmotic stress in a cAMP- and microtubule-dependent fashion. This phenomenon was functionally significant, since aquaporin and microtubule inhibitors reduced the rate of volume recovery. Isolated contractile vacuoles were enriched in alkaline phosphatase, aquaporin, and some acidocalcisomal markers.","abstract_has_math":false,"creators":["Rohloff, Peter John"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Veterinary Pathobiology","degree_department":null,"school":null,"contributors":["Roberto Docampo"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-28T16:21:36Z","date_published":"2015-09-28T16:21:36Z","updated_at":"2026-07-22T22:26:30Z","subjects":["Health Sciences, Pathology"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3101957"],"render_values":[{"text":"(MiAaPQ)AAI3101957","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/87623","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Roberto Docampo"]},{"key":"dc:creator","label":"Author","values":["Rohloff, Peter John"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-28T16:21:36Z","10000-01-01","2003"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Veterinary Pathobiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Health Sciences, Pathology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/87623","(MiAaPQ)AAI3101957"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Electron microscopic observations revealed a contractile vacuole coupled to acidocalcisomes in all three life cycle stages of the parasite. 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