Directed Evolution of T Cell Receptor Antagonists

In Chapter 4, further engineering of TCR stability mutants to produce increased affinity binding for SAg is described. An optimized TCR fragment (containing nine mutations) was isolated after mutagenesis and selection, and was found to have approximately 1000-fold improved affinity for SAg. A soluble form of the mutant was shown to completely and specifically inhibit T cell activity in vitro.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Kieke, Michele Catherine

Contributors dc:contributor

• Kranz, David M.

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(MiAaPQ)AAI9996644

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/86768