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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 158 for “"Directed Evolution"”.
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Directed evolution of antimutator E. coli
… systems – cells are tiny factories that evolve. Evolution poses a problem because when a desired function is encoded into the DNA of the host organism, the host uses its own resources to perform the function and there is likely no associated fitness benefit. Therefore, there is strong selection …
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Directed evolution of split APEX peroxidase
… most optimal cut site (Chapter 2). We then used directed evolution on the top candidate pair to engineer a split APEX tool (sAPEX). Selections were performed via FACS on yeast-displayed fragment libraries, and 20 rounds of evolution produced a 200-amino acid Nterminal fragment (with 9 mutations …
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Continuous Directed Evolution in Mammalian Cells
Directed evolution is a powerful methodology for the creation of new biomolecules with user-desired functions. Most directed evolution experiments are performed in vitro, in bacteria, or in yeast, even when the evolved biomolecule is intended to function in mammalian cells. As a result, the …
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Directed Evolution of Glycan-Binding Proteins
… small, hyperthermostable DNA binding protein by directed evolution. A yeast surface display method for evolution of GBPs was developed and used to generate GBPs for the recognition of mammalian glycans sialic acid and the cancer-associated disaccharide Thomsen-Friedenreich (TF) antigen. …
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Directed Evolution of T Cell Receptor Antagonists
In Chapter 4, further engineering of TCR stability mutants to produce increased affinity binding for SAg is described. An optimized TCR fragment (containing nine mutations) was isolated after mutagenesis and selection, and was found to have approximately 1000-fold improved affinity for SAg. A …
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DIRECTED EVOLUTION FOR THE DESIGN OF NEW CATALYSTS
… De novo computational design coupled with directed evolution can yield catalysts with novel functions and improved catalytic rate enhancement of enzymes.1-2</p> <p>Minimalist protein design that focuses on the bare minimum requirements to achieve activity presents several important …
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New strategies for in vivo continuous directed evolution
Continuous in vivo directed evolution facilitates the exploration of large biomolecule libraries at unprecedented speeds. By inserting a biomolecule of interest into a constantly mutating virus whose replicative capacity has been rendered dependent on the desired activity of that biomolecule, the …
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Directed evolution in human cells via adenoviral replication
… development. Typically, researchers turn to directed evolution either in vitro, in bacteria, or in yeast, to mutate, select, and amplify biomolecules of interest (BOls) with new and highly-tailored activities. Unfortunately, BOIs evolved in these environments often fail to function when …
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Directed Evolution of Macrocyclic Peptides For Inhibition of Autophagy
<p>In recent decades it has become increasingly clear that induction of autophagy plays an important role in the development of treatment resistance and dormancy in many cancer types. Chloroquine (CQ) and hydroxychloroquine (HCQ), two autophagy inhibitors in clinical trials, suffer from poor …
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Enzyme and Directed Evolution Technologies For Nerve Agent Neutralisation
… successful approach has been the application of directed-evolution strategies. The aim of the present work has been to create variants of PTE with an increased catalytic efficiency towards OPs nerve agents. To this end, a directed evolution platform was developed to enable screening for …
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Towards biosensor-assisted directed evolution of myo-inositol oxygenase
… of the biosensor for this work was the directed evolution of myo-inositol oxygenase (MIOX), the enzyme that catalyzed the penultimate, rate-limiting step in the metabolic production of glucarate from glucose in E. coli. Here, we develop a biosensor that recognizes glucuronate, the direct …
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Quantitative methods for multiplexed cellular engineering and directed evolution
… system to guide the design of proteins in directed evolution experiments and is generally applicable to all multiplexed library screens.
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Engineering a highly enantioselective horseradish peroxidase by directed evolution
… aforementioned method, in just three rounds of directed evolution we both greatly increased (up to 30-fold) and also reversed (up to 70-fold) the enantioselectivity of the commercially useful enzyme, horseradish peroxidase (HRP), toward a chiral phenol. In doing so, we discovered that mutations …
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Engineering spores to display g protein-coupled receptors for directed evolution
… engineering and optimizing membrane proteins by directed evolution.
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Directed Evolution of Phosphite Dehydrogenase and Engineered Biosynthesis of Fr-900098
Another area in which biocatalysts are increasingly being used is in the production of small secondary metabolites, particularly antibiotics. Phosphonates are a small but growing class of compounds with many useful therapeutic properties. In particular, the phosphonates fosmidomycin and FR-900098 …
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Combatting pathogens through directed evolution, single-cell sequencing, and synthetic immunology
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2027-05-01
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Directed evolution of amino acid dehydrogenases for biocatalysis of chiral amines
… Darwinian natural selection in the laboratory, directed evolution has become a powerful practical approach to study enzymes and optimize them to catalyze industrially relevant transformations. In this thesis, I applied this strategy to the engineering of amino acid dehydrogenases for …
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A directed evolution approach to engineering recombinant protein production in S. cerevisiae
The continued success of protein therapeutics has put a strain on industry's ability to meet the large demand. Creating a more productive expression host for the manufacture of these proteins is a potential solution. Although heterologous proteins are frequently made in organisms as disparate as E. …
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