Studies of Acetyl-Coa Carboxylase From Escherichia Coli

Preliminary functional assignments for the two subunits in the carboxyltransferase complex have been given. The alpha subunit (AccA) appears to have a structural role in the total ACC complex while the beta subunit (AccD) could contain the carboxyltransferase active site. The above conclusions are based on enzymatic deactivational studies with extracts only overproducing AccBCD with and without PEG (14%) compared to extracts with all four subunits overproduced (AccABCD) for the assignment of the AccA function. Extracts only overproducing AccD contained higher carboxyltransferase activity leading one to conclude the active site for the complex is contained in the AccD subunit.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Davis, Mark Stephen

Contributors dc:contributor

• Cronan, John E., Jr

• Biology, Molecular

Language dc:language

eng

Identifier

(MiAaPQ)AAI9912216

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/86735