Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 52 for “"alpha subunit"”.
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Integrin Alpha Subunit Expression and Cytoplasmic Domain Function
The $\beta\sb1$ integrins are a family of $\alpha\beta$ heterodimeric cell surface receptors which mediate cell adhesion to molecules of the extracellular matrix (ECM), and also span the membrane and associate with the cytoskeleton via their cytoplasmic domains. By mediating the interaction of the …
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Characterization of Gf a Drosophila trimeric G protein alpha subunit
… thesis work, I address the role of a trimeric G alpha protein in Drosophila, Gαf, and what part it may play in development. In particular, I explore the role of Gαf as an alpha subunit of a trimeric complex, to determine what heptahelical receptors might act as its cognate receptor.
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Identification of Phosphorylase Kinase Alpha Subunit Binding Partners in Skeletal Muscle
<p>IDENTIFICATION OF PHOSPHORYLASE KINASE ALPHA SUBUNIT BINDING PARTNERS IN SKELETAL MUSCLE Soleil Archila August 2004 67 Pages Directed by: Nancy A. Rice, Sigrid Jacobshagen, and Claire A. Rinehart Department of Biology Western Kentucky University Phosphorylase kinase (PhK) integrates neural, …
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Dual Mechanisms Regulating Alpha Subunit-Specific Activity in Hypoxia-Inducible Factor Signaling
The ability to adapt to and protect from environmental stresses is essential to survival and has played a major role in fitness selection during evolution. As oxygen is essential to most life, many organisms have developed a response to conditions of low oxygen availability. Throughout the animal …
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Cholesterol Modulation of BK Currents and Cerebral Artery Diameter Via Channel-Forming Alpha-Subunit
… to specific amino acid(s) of the channel-forming alpha subunit, and involving distinct modifications of channel gating. This project determines that cholesterol binds to the protein at physiologically relevant levels. A single amino acid, Y450, is required for this interaction between cholesterol …
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Integrin Alpha Subunit Ratios, Cytoplasmic Domains, and Growth Factor Synergy Regulate Muscle Proliferation, Differentiation, and Signaling
… was addressed by ectopic expression of integrin alpha subunits in primary quail skeletal muscle. Ectopic expression of either the human or $\alpha$5 or the chicken $\alpha$6 subunit produced contrasting phenotypes. The $\alpha$5 transfected myoblasts remain in the proliferative phase and are …
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Regulation of Guanine Nucelotide Exchange in Inhibitory G Protein Alpha Subunit by Activator of G Protein Signaling 3 and Novel Regulatory Peptides
… the nucleotide binding pocket of G protein a subunit (Ga) is accelerated by guanine nucleotide exchange factors (GEFs) and inhibited by guanine nucleotide dissociation inhibitors (GDIs). The beta gamma subunit of heterotrimeric G protein and GoLoco motif are GDIs. Activator of G protein …
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Characterization of proteins purified from a beta(1) integrin affinity column: A novel ecm molecule and a muscle-specific integrin alpha subunit
… novel integrin-associated proteins or even new $\alpha$ subunits motivated this work which was to characterize two of the major proteins with the molecular weights at 220 kD and 70 kD, respectively.
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Modulation of Alpha-Subunit VISIT-DG Sequence Residues Ser-347, Gly-351 and Thr-349 in the Catalytic Sites of <em>Escherichia coli</em> ATP Synthase.
… we investigate the role of highly conserved α-subunit VISIT-DG sequence residues αSer-347, αGly-351, and αThr-349 in P<sub>i</sub> binding. Mutations αS347A/Q, αG351Q, αT349A/D/R, βR182A, and αT349R/βR182A were generated via site directed mutagenesis. Results from biochemical assays showed …
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Genetic analysis of RNA polymerase/transcriptional activator interactions at thepepT promoter of S. typhimurium
The role of the alpha subunit of RNA polymerase (encoded by rpoA) in transcriptional activation by the global regulatory protein FNR has been investigated using a genetic approach. We have identified five rpoA mutations which decrease the FNR-dependent anaerobic induction of the pepT gene of S. …
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Bacterial Luciferase: Studies of Proteolytic Inactivation and Ligand Binding
… structure; and (4) the fragmentation of the (alpha) subunit into a discrete series of peptides, denoted the (gamma) and (delta) families. The region of the (alpha) subunit which was susceptible to proteolytic clipping was termed the Proteinase Labile Region and was observed to be a conserved …
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Studies of Acetyl-Coa Carboxylase From Escherichia Coli
Preliminary functional assignments for the two subunits in the carboxyltransferase complex have been given. The alpha subunit (AccA) appears to have a structural role in the total ACC complex while the beta subunit (AccD) could contain the carboxyltransferase active site. The above conclusions are …
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VOLTAGE GATED SODIUM CHANNEL AND NEURONAL DIFFERENTIATION. IMPLICATIONS IN AMYOTROPHIC LATERAL SCLEROSIS PATHOGENESIS
… are multimeric protein complexes formed by one alpha subunit, responsible of pore formation and of the regulation of the state of the channel, and by one or two accessory beta subunits that have an important role in the regulation of alpha subunit localization and kinetics and in the interaction …
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Understanding the function of ICOS/ICOSL costimulation in experimental autoimmune myasthenia gravis
… stimulation with AChR or the immunodominant AChR alpha-subunit peptide 146-162. In vivo, the lack of ICOS costimulation led to diminished B cell and plasma cell expansion, whereas the number of CD4+ T helper cells was increased. Collectively, these results indicate that lymphocyte costimulation …
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Active Center Studies on Bacterial Luciferase: Modification of the Enzyme With 2,4-Dinitrofluorobenzene
… dinitrophenyl moiety is incorporated into the (alpha) subunit approximately twice as fast as it is incorporated into the (beta) subunit; the rate of inactivation is nearly identical to the rate of incorporation into the (alpha)(beta) dimer. The incorporation of 1 mole DNP/(alpha)(beta) results …
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The identification of chemical compounds that decrease cellular levels of toxic Huntington's disease protein through a novel cell-based assay
… (23Q) Htt fused to the [beta]-galactosidase alpha-subunit ([alpha]) in an inducible fashion in PC12 cells which also expressed the [beta]-galactosidase delta-subunit ([delta]). Complementation between these expressed subunits allowed the formation of functional P-galactosidase. The level of …
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Impaired N-Glycosylation Processing Impacts Neuronal Function
… performed by genes known as Mgats. Kv3.1b is an alpha subunit of a voltage-gated potassium channel known as Kv3 channels. Kv3 channels have the ability to open and close much more rapidly than other K channels, thus allowing neurons to create trains of action potentials. Kv3.1b is N-glycosylated …
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Phenotypic diversity as a tool to guide and optimize random strain improvement approaches
… phenotypes of interest. We also considered the alpha subunit of the RNA polymerase as a tool for phenotypic alteration, and fruitfully used it to improve butanol and solvent tolerance, accumulation of hyaluronic acid, and L-tyrosine production.
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Daple Orients and Patterns Sensory Hair Bundles in the Inner Ear
… by a G-protein complex comprising the alpha subunit of heterotrimeric G-protein (Gαi) and Leu-Gly-Asn repeat-enriched protein (LGN). Classically known for orienting the mitotic spindle, these proteins form an apical blueprint for hair-bundle development. Although the primary cilium also …
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Active Center Structure and Sequence Studies on Bacterial Luciferase Utilizing the Essential Cysteine, Protease-Labile Region, and Delta Fragment
… two well-documented structural features of the (alpha) subunit of the enzyme, the essential cysteine and the protease-labile region, which are thought to reside there. Chemical modification of this cysteinyl residue causes slight perturbations, dependent upon the structure of the modifying …
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