Molecular and Genetic Characterization of SAS Genes in Position Effect Silencing in Saccharomyces Cerevisiae

Further characterization of SAS4 and SAS5 demonstrates that they define a new SIR-dependent silencing pathway in yeast. SAS4 and SAS5 are required for silencing at teloments while SIR1 is not. Also, SAS4 and SAS5 have opposite phenotype from SIR1 at HMR. These results indicate that SAS4 and SAS5 can function on a SIR1-independent silencing pathway. Furthermore, SAS4 and SAS5 contribute to silencing at HML. Double mutant analysis of silencing at HML between SAS4, SAS5 and SIR1 suggests that SAS4 and SAS5 may have similar function as SIR1 acting in establishment of silencing. Taken together, these evidence demonstrates that there exist multiple SIR-dependent pathways of silencing in yeast, which provide first genetic evidence of existence of multiple pathways of positon effects in any eukaryote.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Xu, Eugenia Yujin

Contributors dc:contributor

• David H. Rivier

• Biology, Molecular

Language dc:language

eng

Identifier

(MiAaPQ)AAI9904629

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/86733