Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"mutant analysis"”.
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Mutant analysis of luminescence and autoinduction in a marine bacterium
… of luminescence. Most dramatically, the mutants revealed the presence of multiple autoinducers and autoinducer synthases in V. fischeri. One of the autoinducers (autoinducer-2, or AI-2) was chemically purified and shown to be Noctanoyi- L-homoserine lactone. The genetic locus encoding the …
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Mutant analysis of luminescence and autoinduction in a marine bacterium
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1995.
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Molecular and Genetic Characterization of SAS Genes in Position Effect Silencing in Saccharomyces Cerevisiae
… and SAS5 contribute to silencing at HML. Double mutant analysis of silencing at HML between SAS4, SAS5 and SIR1 suggests that SAS4 and SAS5 may have similar function as SIR1 acting in establishment of silencing. Taken together, these evidence demonstrates that there exist multiple SIR-dependent …
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Molecular and Genetic Analysis of the Role of Ku in Silencing and Telomere Function in Saccharomyces Cerevisiae
… function, we performed a Synthetic Genetic Array analysis focusing on genes with roles in silencing (YKU70, SIR1, SAS2, SAS5, SET1, ASF1) and telomere length regulation (YKU70, BDF1, ANC1, RAD50, TEL1, RAD52). Double mutant analysis revealed functional relationships between genes by generating a …
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Scaffold-Mediated Organization of Signal Transduction Networks
… loss-of-function and partial loss-of-function mutant analysis, we demonstrate that Raf can participate in cell regulatory processes independently of its ability to activate MEK. We also show that scaffolding proteins can serve as specificity determinants in Ras/MAPK signaling, and may function …
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Odorant Responses and Courtship Behaviors Influenced by at4 Neurons in Drosophila
… Using a combination of odorant receptor mutant analysis, single sensillum electrophysiology and optogenetics, I have examined the chemical tuning and behavioral consequences of the three at4 olfactory neuron classes. My results indicate that one class, Or65abc neurons, previously reported …
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Establishing a positional information assay for brain ventricle mutants and investigating the choroid plexuses in zebrafish
… ventricles can be understood through genetic mutant analysis. Within the framework of a characterization of zebrafish mutants with brain ventricle phenotypes, I have developed an assay that looks for evidence of compromised gene expression patterns. I have shown that a cocktail of krox20, …
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Identification and analysis of Arabidopsis thiamine pyrophosphate transporters
… the aims of this project include phylogenetic analysis, mutant analysis, polymerase chain reaction techniques and gene construct techniques. AtTpc1 and AtTpc2 have a limited effect on plant growth under normal conditions, at least in terms of stem length and secondary stem length. Moreover, …
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P125, A COPII INTERACTING PROTEIN
… study, using GST-pulldown and mass spectrometry analysis, p125A is also identified as one of the proteins interacting with Sec31A, another member of the COPII coat. Deletion mutant analysis of p125A narrowed its Sec31A-interacting domain to be within residues 260-600. Binding of p125A to Sec31A …
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Sexual Reproduction and Signal Transduction in the Candida Species Complex
… these organisms has been significantly rewired. Mutant analysis of the retained transcription factors is used to understand how sexual identity and sporulation are controlled in these strains. Secondly, based on the observation that these species are missing many key genes involved in mating and …
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Drosophila Cyclin J And The Somatic Pirna Pathway Cooperate To Regulate Germline Stem Cells
… under normal conditions. Further double mutant analysis of CycJ and neighbor armi, as well as two other piRNA pathway members (piwi and aub), revealed that CycJ genetically interacts with the piRNA pathways by enhancing their ability to regulate oogenesis. Double mutants of CycJ and …
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Growth Cone Pathfinding and Neuromuscular Synaptogenesis in Drosophila Midline Mutants
… embryo and high resolution single cell analysis, I examined the function of commissureless, netrin, roundabout, and frazzled during neural development. Analysis of the individual axon pathways of RP3, V, and RP2 in wildtype and commissureless and roundabout mutants investigated how …
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Potential Roles of Peroxidases In Caenorhabditis Elegans Innate Immunity
… to BLI-3.</p> <p>By RNAi and <em>skpo-1</em> mutant analysis, I determined that SKPO-1 is involved in the host immune response during <em>E. faecalis</em> infection. By tissue-specific RNAi, I determined that SKPO-1 is functionally active in the hypodermis and required for wild type resistance …
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Conformational Rearrangements In The Sensory Rcsf/Omp Complex Mediate Signal Transduction Across The Bacterial Cell Envelope
… isolated <em>rcsF</em> and <em>bamA</em> mutants that impact RcsF/BamA and RcsF/OMP interaction, I was able to test several proposed models for Rcs signaling. The analysis of these mutants showed that Rcs activation in <em>bam</em> mutants results from secondary OM and lipopolysaccharide …
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Characterization of a fatty acid elongase condensing enzyme by site-directed mutagenesis and biochemical analysis
… potential role in the condensation reaction. Mutant EloAs were expressed in yeast and fatty acid methyl esters prepared from total cellular lipids were analyzed by gas chromatography/mass spectrometry. Sixteen out of twenty mutants had a decrease in 18:1Δ11 production when compared to the …
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Genetic and Cytological Analysis of the Spindle Assembly Checkpoint Component BUB-3 in DNA Damage Response
… in the DNA damage response. bub-3(gt2000) mutants are moderately sensitive to the crosslinking drug cisplatin but not to UV light or methyl methanesulfonate. This is consistent with a role in dealing with DNA double-strand breaks and not with base damage. bub-3 and san-1 mutants did not …
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Isolation and Characterization of Two siamese Phenotypic Modifiers and Their Role in Endoreplication and Trichome Development
… in ENS1 also enhance the multicellularity of sim mutant trichomes indicating that ENS1 plays a role in the regulation of the cell cycle during trichome cell differentiation. We have shown that ENS1 is required by the negative regulators of trichome branching for the trichomes to achieve their …
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Regulation of Tubulin dynamics by the +Tip tracking protein Mal3
… pull-down of the endogenous complex and analysis of its constituents by mass spectrometry revealed that Tea2 and Tip1 form a tight stoichiometric complex, making a much more labile interaction with Mal3. Biochemical experiments, light scattering and DIC microscopy demonstrate that Mal3 …
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Functional and expression analysis of heteromeric acetyl-CoA carboxylase subunit genes of Arabidopsis
… by real-time RT-PCR and quantitative western analysis. At the mRNA level, CAC1-A, CAC2, CAC3, and accD genes are expressed at a constant molar ratio of 0.5:1.0:0.2:2.0 across all the organs examined, but the expression pattern of CAC1-B is different. At the protein level, there is no …
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