The Roles of Peroxynitrite and Superoxide in Oxidative Damage to E. Coli

Peroxynitrite (ONOO$\sp{-}),$ a toxic biomolecule produced from the reaction of superoxide and nitric oxide (NO$\cdot)$ in the phagolysosome, may also be toxic by the same mechanism as O$\sb2{-}.$ Our in vivo results indicated that peroxynitrite has the same intracellular targets as superoxide. Of nineteen enzymes tested, only the dehydratase enzymes containing these iron-sulfur clusters were significantly affected by peroxynitrite challenge. These iron-sulfur clusters could be repaired, and both iron storage proteins and iron import seem to be necessary for efficient repair. This enzyme inactivation led to an increased intracellular free iron. However, unlike superoxide. the free iron was rapidly sequestered.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Opperman, Kay Keyer

Contributors dc:contributor

• Imlay, James A.

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(MiAaPQ)AAI9812724

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/86724