Studies of Lipoic Acid Related Enzymes

Using a similar strategy of library construction and screening, I also isolated the Vibrio harveyi acyl-ACP synthetase gene and showed that it encoded a 60.4 KDa protein that shared high homology with medium chain fatty acid-CoA ligases. I expressed the His-tagged protein at a high level in E. coli and purified it to homogeneity. To explore the physiological application of this enzyme in E. coli fatty acid metabolism, I constructed the first E. coli fabH (beta-ketoacyl-ACP synthetase III) bypass mutant in the presence of acyl-ACP synthetase and exogenous fatty acids.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Jiang, Yanfang

Contributors dc:contributor

• Cronan, John E., Jr

• Biology, Microbiology

Language dc:language

eng

Identifier

(MiAaPQ)AAI3182290

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/86677