Cloning, Phenotypic, and Experimental Analysis of Pat-4: The Caenorhabditis Elegans Homologue of Integrin -Linked Kinase

"The third chapter describes experimental analysis of pat-4 during the morphogenesis of the excretory canal cell. By completely removing pat-4 expression from the excretory cell, I demonstrate that pat-4 is essential for the active outgrowth and complete extension of the excretory canal processes during embryogenesis and early larval development. Interestingly, ""kinase-dead"" and PH domain-disrupted forms of PAT-4/ILK support initial canal outgrowth. However, these canals frequently fail to fully extend during active canal outgrowth resulting in shortened, misshaped mature canals. These results identify a new developmental function for PAT-4/ILK in C. elegans and directly establish a role for ILK during the dynamic process of cellular morphogenesis in vivo ."

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Mackinnon, Alexander Craig, Jr

Contributors dc:contributor

• Benjamin D. Williams

• Biology, Cell

Language dc:language

eng

Identifier

(MiAaPQ)AAI3153370

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/86673