Molecular Profiling: Gene Expression Reveals Discrete Phases of Lens Induction and Development in Xenopus Laevis

"From these genes, two were examined to further characterize their roles in development and transdifferentiation. The matrix metalloproteinase Xmmp-9 was found to function during migratory and morphogenetic processes during development, and it was found to be upregulated during wound healing. Psf2 codes for a factor which functions during DNA replication as a member of the GINS heterotetramer (Kubota et al., 2003), a ""sliding clamp"" involved in DNA replication. In situ hybridization revealed that Psf2 is expressed during embryonic development in a tissue-specific manner, including the optic cup and the lens. Morpholino-mediated knockdown of Psf2 resulted in dosage-dependent phenotypes which included microphthalmia, coloboma, and abnormal lens formation. Additionally, tissue manipulation experiments in conjunction with morpholino injections demonstrated intrinsic roles for Psf2 in retinal and lens development and, secondarily, during the late phase of lens induction."

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Microbiology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Walter, Brian Edward

Contributors dc:contributor

• Henry, Jonathan J.

• Biology, Zoology

Language dc:language

eng

Identifier

(MiAaPQ)AAI3131040

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/86667