{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/86667"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/86667","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Molecular Profiling: Gene Expression Reveals Discrete Phases of Lens Induction and Development in Xenopus Laevis","abstract":"\"From these genes, two were examined to further characterize their roles in development and transdifferentiation. The matrix metalloproteinase Xmmp-9 was found to function during migratory and morphogenetic processes during development, and it was found to be upregulated during wound healing. Psf2 codes for a factor which functions during DNA replication as a member of the GINS heterotetramer (Kubota et al., 2003), a \"\"sliding clamp\"\" involved in DNA replication. In situ hybridization revealed that Psf2 is expressed during embryonic development in a tissue-specific manner, including the optic cup and the lens. Morpholino-mediated knockdown of Psf2 resulted in dosage-dependent phenotypes which included microphthalmia, coloboma, and abnormal lens formation. Additionally, tissue manipulation experiments in conjunction with morpholino injections demonstrated intrinsic roles for Psf2 in retinal and lens development and, secondarily, during the late phase of lens induction.\"","abstract_html":""From these genes, two were examined to further characterize their roles in development and transdifferentiation. The matrix metalloproteinase Xmmp-9 was found to function during migratory and morphogenetic processes during development, and it was found to be upregulated during wound healing. Psf2 codes for a factor which functions during DNA replication as a member of the GINS heterotetramer (Kubota et al., 2003), a ""sliding clamp"" involved in DNA replication. In situ hybridization revealed that Psf2 is expressed during embryonic development in a tissue-specific manner, including the optic cup and the lens. Morpholino-mediated knockdown of Psf2 resulted in dosage-dependent phenotypes which included microphthalmia, coloboma, and abnormal lens formation. Additionally, tissue manipulation experiments in conjunction with morpholino injections demonstrated intrinsic roles for Psf2 in retinal and lens development and, secondarily, during the late phase of lens induction."","abstract_has_math":false,"creators":["Walter, Brian Edward"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Microbiology","degree_department":null,"school":null,"contributors":["Henry, Jonathan J."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-28T15:17:17Z","date_published":"2015-09-28T15:17:17Z","updated_at":"2026-07-22T22:26:27Z","subjects":["Biology, Zoology"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3131040"],"render_values":[{"text":"(MiAaPQ)AAI3131040","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/86667","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Henry, Jonathan J."]},{"key":"dc:creator","label":"Author","values":["Walter, Brian Edward"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-28T15:17:17Z","10000-01-01","2004"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Microbiology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Zoology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/86667","(MiAaPQ)AAI3131040"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["\"From these genes, two were examined to further characterize their roles in development and transdifferentiation. The matrix metalloproteinase Xmmp-9 was found to function during migratory and morphogenetic processes during development, and it was found to be upregulated during wound healing. Psf2 codes for a factor which functions during DNA replication as a member of the GINS heterotetramer (Kubota et al., 2003), a \"\"sliding clamp\"\" involved in DNA replication. In situ hybridization revealed that Psf2 is expressed during embryonic development in a tissue-specific manner, including the optic cup and the lens. Morpholino-mediated knockdown of Psf2 resulted in dosage-dependent phenotypes which included microphthalmia, coloboma, and abnormal lens formation. Additionally, tissue manipulation experiments in conjunction with morpholino injections demonstrated intrinsic roles for Psf2 in retinal and lens development and, secondarily, during the late phase of lens induction.\"","Made available in DSpace on 2015-09-28T15:17:17Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3131040.pdf: 9541771 bytes, checksum: 77269af0b4f51b5d3dfb1e5cc0191cea (MD5) Previous issue date: 2004","Embargo set by: Seth Robbins for item 87948 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","147 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2004."]},{"key":"dc:title","label":"Title","values":["Molecular Profiling: Gene Expression Reveals Discrete Phases of Lens Induction and Development in Xenopus Laevis"]}]}],"canonical_facts":{"dc:contributor":["Henry, Jonathan J."],"dc:creator":["Walter, Brian Edward"],"dc:date":["2015-09-28T15:17:17Z","10000-01-01","2004"],"dc:description":["\"From these genes, two were examined to further characterize their roles in development and transdifferentiation. The matrix metalloproteinase Xmmp-9 was found to function during migratory and morphogenetic processes during development, and it was found to be upregulated during wound healing. Psf2 codes for a factor which functions during DNA replication as a member of the GINS heterotetramer (Kubota et al., 2003), a \"\"sliding clamp\"\" involved in DNA replication. In situ hybridization revealed that Psf2 is expressed during embryonic development in a tissue-specific manner, including the optic cup and the lens. Morpholino-mediated knockdown of Psf2 resulted in dosage-dependent phenotypes which included microphthalmia, coloboma, and abnormal lens formation. Additionally, tissue manipulation experiments in conjunction with morpholino injections demonstrated intrinsic roles for Psf2 in retinal and lens development and, secondarily, during the late phase of lens induction.\"","Made available in DSpace on 2015-09-28T15:17:17Z (GMT). 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