Regulation of Gene Transcription by Estrogen Receptor Alpha: The Role of Cis Elements and Trans -Acting Factors

The differential recruitment of coregulatory proteins to DNA-bound ERalpha plays a critical role in mediating estrogen-responsive gene expression. We previously isolated and identified retinoblastoma-associated proteins 46 (RbAp46) and 48 (RbAp48), which are associated with chromatin remodeling, histone deacetylation, and transcription repression as proteins associated with the DNA-bound ERalpha. We have demonstrated that RbAp46 and RbAp48 interact with ERalpha in vitro and in vivo, associate with ERalpha at endogenous estrogen-responsive genes, and alter expression of endogenous ERalpha-activated and -repressed genes in MCF-7 breast cancer cells. Our findings reveal that RbAp48 limits expression of estrogen-responsive genes and that RbAp46 attenuates and enhances estrogen responsiveness in a gene-specific manner. The ability of the transacting factors RbAp46 and RbAp48 to interact with ERalpha and influence its activity reveals yet another role for these multifunctional proteins in regulating gene expression.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Cell and Developmental Biology

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Creekmore, Amy

Contributors dc:contributor

• Clayton, David F.
• Nardulli, Ann M.

• Biology, Cell

Language dc:language

eng

Identifier

(MiAaPQ)AAI3314753

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/86312