Effects of Heterodera Glycines Population Densities on Fusarium Solani F. Sp. Glycines Colonization of Soybean Roots and Development of Sudden Death Syndrome

Estimation of root colonization by Fsg with the plating bioassay was difficult and the results were often unsatisfactory in these experiments. Therefore, an alternative method was explored, relative quantification with real-time quantitative PCR (QPCR), to estimate fungal colonization of soybean roots. QPCR was more sensitive than the plating bioassays at detecting the fungus: Fsg DNA was detected in 371 out of 372 root samples with QPCR, while CFU were only detected in 14.5% of the samples with the plating bioassay. The plating bioassay required at least 10 days longer to complete and the results had higher variability than those obtained with QPCR. Overall, QPCR had many advantages over the plating bioassay and has the potential for several more practical applications.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Crop Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Jackson, Tamra A.

Contributors dc:contributor

• Niblack, Terry L.

• Biology, Microbiology

Language dc:language

eng

Identifier

(MiAaPQ)AAI3182286

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/85020