A Genomic Assessment of Vicia Villosa Roth: Evaluation by Flow Cytometry, Canonical Discriminant Analysis, and Replicated cDNA Microarray Analysis

The final research objective is to determine statistically significant expressed genes in hairy vetch that are also biologically significant to traits hypothesized to be integral in hairy vetch improvement. The RNA of four targets were hybridized to 142 cDNA clones of biotic stress genes and gene sequences of soybean that were robotically spotted onto aminosilicated slides. The microarray data was analyzed using the individual fluorescence intensities to fit two statistical models with a mixed model analysis of variance using Proc Mixed in SAS. Ninety-seven of the 142 gene sequences (68%) were differentially expressed in at least one of the target comparisons. Eighty-three of the sequences (58%) were differentially expressed in hairy vetch. Among these are two sequences that encode for proline-rich proteins, which are abundant in cell wall structures and are involved in plant development.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Crop Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Yeater, Kathleen Mary

Contributors dc:contributor

• Bollero, German A.

• Agriculture, Agronomy

Language dc:language

eng

Identifier

(MiAaPQ)AAI3086221

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/85011