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University of Illinois at Urbana-Champaign
DNA and Ligand Binding and Kinetic Properties of Wild-Type and Mutant RsrI [n6-Adenine] DNA Methyltransferases
Abstract
dc:descriptionTo complement the biochemical characterization of M•Rsr I in vitro, DNA binding analysis in vivo was performed using the bacteriophage P22-based challenge-phage assay. I isolated a catalytically impaired mutant, M•RsrI (L72P), that, unlike wild-type M•RsrI, binds in a methylation-sensitive, site-specific manner to DNA.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Biochemistry
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2015
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Szegedi, Sandra Suzanne
- Contributors dc:contributor
-
- Gumport, R.I.
Subjects
dc:subject × 1Rights
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
- (MiAaPQ)AAI9971200
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/84915