Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 99 for “"methyltransferases"”.
-
The Role of tRNA methyltransferases in Cancer
RNA modifications have emerged as pivotal regulators of cellular homeostasis, stress adapta- tion, and disease, particularly within the context of cancer. Among these, tRNA modifications play a crucial role in maintaining translational fidelity and supporting cancer cell survival under adverse …
-
Role of RNA methyltransferases in lung cancer
… intervention. Here, I target validated two RNA methyltransferases. I performed a late-stage study of METTL3, and I initiated and developed an early-stage analysis of THUMPD3. Advanced validation of METTL3, a well characterised m⁶A RNA methyltransferase, was performed based on the availability of …
-
Phosphoethanolamine methyltransferases inhibitors with broad-spectrum anthelmintic effect against livestock nematodes
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-08-01
-
DNA Methylation and DNA Methyltransferases in the Clonal Raider Ant, Ooceraea biroi
<p>DNA methylation and DNA methyltransferase enzymes (DNMTs) are ubiquitous and predate the origin of eukaryotes. In animals, DNA methylation primarily is carried out by DNMT1, which targets hemi – methylated DNA and maintains methylation patterns through cycles of cell replication, and DNMT3, the …
-
Release of Histone Lysine Methyltransferases from Rat Brain Chromatin by Nuclease Digestion
<p>The histone lysine methyltransferases catalyze the transfer of methyl groups from S-adenosyl-L-methionine to specific ε-N-lysyl residues in the N-terminal regions of histones H3 and H4. These enzymes are located exclusively within the nucleus and are firmly bound to chromatin. The …
-
Characterization of mutant N², N²-dimethylguanosine-specific tRNA methyltransferases from Saccharomyces cerevisiae
$N\sp2,N\sp2$-dimethylguanosine-specific tRNA methyltransferase (m$\sbsp{2}{2}$Gtase) is an enzyme which uses S-adenosylmethionine as a substrate to catalyze the transfer of two methyl groups to the 2-amino group of guanosine at position 26 in tRNA. Neither the tertiary structure nor the location …
-
Synthesis, Screening and Cocrystallization of Adenosine Based Inhibitors with Methyltransferases, ErmC' and KsgA
… dimethylating A1518/A1519 of 16S rRNA. Like most methyltransferases, ErmC’ and KsgA use cofactor S-adenosylmethionine (SAM) as their methyl source, which binds adjacent to their specific adenosine substrate. ErmC’ inhibitors could restore MLSB antibiotics against infections with this resistance …
-
Using "Designer" Nucleosomes to Study Enzymatic Crosstalk Between Histone Ubiquitylation and Histone Methyltransferases
… It has been previously shown that H3 lysine 4 methyltransferases involved in transcriptional activation are stimulated and repressed by H2Bub and H2Aub, respectively. Here, using chemically-defined "designer" mononucleosomes, I tested whether nucleosomal H2BK120ub and H2AK119ub influence the …
-
DNA and Ligand Binding and Kinetic Properties of Wild-Type and Mutant RsrI [n6-Adenine] DNA Methyltransferases
To complement the biochemical characterization of M•Rsr I in vitro, DNA binding analysis in vivo was performed using the bacteriophage P22-based challenge-phage assay. I isolated a catalytically impaired mutant, M•RsrI (L72P), that, unlike wild-type M•RsrI, binds in a …
-
Thermodynamic and Structural Characterization of the Interaction between Wd40 Repeat Containing Protein 5 (Wdr5) and Human Set1 Family Methyltransferases
… of the SET1 family of histone H3 lysine 4 (H3K4) methyltransferases, which are required for transcription of genes involved in hematopoiesis and development. MLL1 associates with a sub-complex containing WDR5, RbBP5, Ash2L, and DPY-30 (WRAD), which together form the MLL1 core complex that is …
-
The γ-tocopherol-like family of N-methyltransferases: A taxonomically clustered gene family encoding enzymes responsible for N-methylation of monoterpene indole alkaloids
… biosynthesis, revealed eight new homologous methyltransferases. This thesis describes the identification, molecular cloning, recombinant expression and biochemical characterization of two picrinine NMTs, one from V. minor and one from R. serpentina, a perivine NMT from C. roseus, and an …
-
Functional genomics through metabolite profiling and gene expression analysis in Arabidopsis thaliana
… the Arabidopsis genome are 24 related carboxyl methyltransferases that belong to the SABATH family. Several of the SABATH methyltransferases convert plant hormones, like jasmonic acid, indole-3-acetic acid, salicylic acid, gibberellins, and other plant constituents into methyl esters, thereby …
-
Design and synthesis studies of new s-adenosyl-l-methionine analogues
… and methionine catalyzed by adenosyltransferase. Methyltransferases (MTs) transfer the activated methyl group from the sulfur center to a specific position in a variety of substrates, e.g., DNA, RNA, proteins, and secondary metabolites. Methyltransferases have been linked to various diseases, …
-
Structures and Regulation of the N6-Methyladenosine Methyltransferase METTL16
… and decay. The m6A modification is deposited by methyltransferases using S-adenosylmethionine (SAM) as the methyl donor. Determining the specificity and mechanism of action of m6A methyltransfersaes is an essential step in dissecting the role of the m6A modification in RNA metabolism. Two enzymes …
-
Predicting protein-DNA interactions using statistical modeling and biophysical representations of high-throughput sequencing data
… data. First, we investigate the action of DNA methyltransferases in a yeast species naturally free of DNA methylation. Using bisulfite-seq and RNA-seq, we measure DNA methylation rates and the impact of induced methylation on gene expression. To understand the behavior of DNA methyltransferases …
-
The KsgA methyltransferase: Characterization of a universally conserved protein involved in robosome biogenesis
The KsgA enzymes comprise an ancient family of methyltransferases that are intimately involved in ribosome biogenesis. Ribosome biogenesis is a complicated process, involving numerous cleavage, base modification and assembly steps. All ribosomes share the same general architecture, with small and …
-
Mechanistic studies of Fusobacterium genetic and defense systems
… systems with a focus on the role of DNA methyltransferases and endonucleases in host defense and genetic recalcitrance in several strains of Fusobacterium through bioinformatic and biochemical approaches. Altogether, over 15 DNA methyltransferases were characterized. Most notably, we …
-
Viengrandines mažąsias RNR modifikuojančių gyvūnų Hen1 metiltransferazių taikymas RNR žymėjimui ir sekoskaitai /
Animal Hen1 methyltransferases are composed of the N-terminal methyltransferase domain and the variable-length C-terminal region, and catalyze the S-adenosyl-L-methionine (AdoMet) dependent methylation of the 2'-O-ribose of the 3'-terminal nucleotide in a subset of small non-coding RNAs. This …
-
Studies of a DNA modification methyltransferase from Rhodobacter sphaeroides
The DNA methyltransferases represent an interesting class of enzymes for the study of protein-DNA interactions due to their high specificity, structural simplicity, and biological importance. RsrI methyltransferase (M$\cdot$RsrI) from Rhodobacter sphaeroides recognizes duplex d(GAATTC) and deposits …
-
TARGETING TYPE-I PRMTS RESTORES PLATINUM SENSITIVITY IN OVARIAN CARCINOMA THROUGH MITOCHONDRIAL METABOLISM
… genotoxic stress, type-I protein arginine methyltransferases–mediated post-translational modifications, and mitochondrial metabolic plasticity underpinning chemoresistance in EOC. We present an integrative multi-omics analysis of carboplatin-resistant A2780 ovarian cancer cells (A2780R) …
Page 1 of 5