Mechanistic Investigations of Dehydration of Lacticin 481 Synthetase and Exploring Its Biochemical Applications

Developing a selective labeling method for peptides/proteins is of great interest in numerous research areas. In order to explore this idea, a strategy was developed through which an N-terminal ketone was introduced by using the LctA-LctM modification system. Finally, analysis of phosphorylated LctA intermediates in collaboration with Professor Kelleher's laboratory revealed dehydration of LctM takes place with directionality, moving from the N-terminus to the C-terminus of the substrates.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Biochemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• You, Young-Ok

Contributors dc:contributor

• van der Donk, Wilfred A.

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(MiAaPQ)AAI3337975

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84858