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Showing 1 to 9 of 9 for “"LctM"”.
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Investigations of the Mechanism and Substrate Scope of the Enzymes Involved in Lantibiotic Biosynthesis
… reconstitution of the lacticin 481 synthetase, LctM, led to preliminary investigations of its low substrate specificity. In this thesis the substrate specificity of LctM was tested further with non-proteinogenic amino acids. The effect of D-amino acids in the truncated prepeptide, LctA, on LctM …
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Investigations of the Substrate Specificity of Lacticin 481 Synthetase and Utilization in Peptide Engineering Applications
The promiscuous activity of LctM toward LctA prepeptides containing nonproteinogenic amino acids prompted the evaluation of LctM as a general catalyst for the introduction of post-translational modifications in non-lantibiotic peptides fused to the LctA leader peptide. LctM was utilized to …
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From Peptide Precursors to Lanthionine -Containing Peptide Antibiotics Using the First Isolated and Active Lacticin 481 Synthetase: Mechanistic Studies and Peptide Engineering
… was investigated. Both the modification enzyme LctM and the substrate LctA were cloned and purified to homogeneity. The in vitro reconstitution of LctM, the lacticin 481 synthetase, was successful. The assay product was characterized in detail through tandem mass spectrometry, suggesting the …
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Investigation of the Lacticin 481 Synthetase and Its Substrate LctA in the Production of the Antibiotic Lacticin 481
… LanM enzyme. Lacticin 481 synthetase (LctM) catalyzes the post-translational modifications required for the generation of the lantibiotic lacticin 481. Previous studies in our laboratory revealed the protein responsible for the creation of the intricate polycyclic antimicrobial agent …
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Amalgamating Information Technology With Library Services In The School Environment: A Learning Commons Transformative Model
… of the Learning Commons Transformative Model (LCTM). The researcher sought to evaluate the newly designed space and the influence it had on teachers’ instruction and subsequent student learning outcomes. This model set the stage for this study by distinctly aligning clear goals with specific …
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Mechanistic Investigations of Dehydration of Lacticin 481 Synthetase and Exploring Its Biochemical Applications
… ketone was introduced by using the LctA-LctM modification system. Finally, analysis of phosphorylated LctA intermediates in collaboration with Professor Kelleher's laboratory revealed dehydration of LctM takes place with directionality, moving from the N-terminus to the C-terminus of the …
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Mechanistic studies of class II lanthipeptide synthetases and yeast surface display of lanthipeptide leader peptides
… ring. By studying truncated enzymes of ProcM, LctM, and CylM, I showed that the N-terminus of these LanMs catalyzed the dehydration and the C-terminal domain catalyzed the cyclization. These two domains were active on their own, indicating that they are relatively independent. The N-terminus of …
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Mechanistic Investigations of Cyclase Enzymes Involved in Lantibiotic Biosynthesis
… Similar studies have been performed using LctM, the LanM protein involved in lacticin 481 biosynthesis. Cysteine and histidine mutants have been generated and analyzed to determine if eliminating the protein's ability to bind zinc leads to a loss in cyclization activity. Results from these …
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Chemical and chemoenzymatic syntheses of lantibiotics and other bioactive cyclic peptides
New antibiotics are desperately needed to combat the disturbing rise of pathogenic microorganisms resistant to traditional treatments. Throughout the history of modern medicine, natural products have played a leading role as a source and inspiration of new drugs, particularly antibacterial agents. …