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University of Illinois at Urbana-Champaign
A Study of the Mechanistic Diversity Inherent to the Ribulose Phosphate Binding Barrel Superfamily
Abstract
dc:descriptionTo probe the functional plasticity of the RPBB superfamily, rational design was used to enhance the UPS activity within the KGPDC scaffold. The triple mutant E112D/T169A/R139V had an enhancement in kcat /KM by 260 fold over wild-type KGPDC. Structural studies suggest that little perturbation of the active site occurs with the 3 mutations.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Biochemistry
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2015
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Akana, Julie A.
- Contributors dc:contributor
-
- Gerlt, John A.
Subjects
dc:subject × 1Rights
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
- (MiAaPQ)AAI3223532
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/84822