Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 33 for “"Phosphate binding"”.
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Phosphatidylinositol 3-phosphate binding properties and autoinhibition mechanism of Phafin2
… PH and FYVE domains bind phosphatidylinositol 3-phosphate [PtdIns(3)P], a phosphoinositide mainly found in endosomal and lysosomal membranes. Phafin2 acts as a PtdIns(3)P effector for endosomal cargo trafficking, macropinocytosis, apoptosis, and autophagy. The PtdIns(3)P binding activity is …
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Binding of organic monophosphates to the dihydroxyacetone phosphate binding sites of rabbit muscle aldolase
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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A Study of the Mechanistic Diversity Inherent to the Ribulose Phosphate Binding Barrel Superfamily
To probe the functional plasticity of the RPBB superfamily, rational design was used to enhance the UPS activity within the KGPDC scaffold. The triple mutant E112D/T169A/R139V had an enhancement in kcat /KM by 260 fold over wild-type KGPDC. Structural studies suggest that little perturbation of the …
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Investigation of Substrate Specificity in Phosphate Binding Barrels and Mechanistic Studies of Orotidine Monophosphate Decarboxylase
"Orotidine 5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum (MtOMPDC) catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) and uridine 5'-monophosphate (UMP) with an enormous rate acceleration, 1.8 x 1016 (kcat = 5 s-1, 25°C; knon = 2.8 x 1016 s-1), and …
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Lanthanide Supramolecular Phosphate Receptors for Environmental and Medical Applications
Phosphate is ubiquitous in all forms of life and the environment. An essential nutrient for plants and humans, it is an increasingly important resource to meet the ever-increasing agricultural demand of modern society. However, excesses of phosphate can cause negative consequences: eutrophication …
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Discovery and Optimization of a Family of Naturally Encoded Antibiotic Compounds that Evade Antibiotic
… evasion is attributed to a dual polyprenyl phosphate binding mechanism. In this thesis, I present discovery and optimization efforts to expand this promising novel class of antibiotic natural products as well as to develop a singular lead clinical drug candidate that displays optimal …
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Molecular Modulation of a-Subunit VISIT-DG Sequence Residue Asp-350 in the Catalytic sites of <em>Escherichia coli</em> ATP Synthase.
… critical amino acid residues involved in Pi binding have to be identified. The αVISIT-DG sequence at the interface of α/β subunits that contains residues from 345-351 is highly conserved and αAsp-350 has been chosen because of its negative charge side chain and its close proximity (~2.8 Å) to …
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Structural requirements for enzymatic efficiency in cofactor-independent decarboxylation
Orotidine 5’-monophosphate decarboxylase (OMPDC) is the last enzyme in the de novo pyrimidine biosynthetic pathway. It catalyzes the decarboxylation of orotidine 5’-monophosphate (OMP) to uridine 5’-monophosphate (UMP), without the requirement of a cofactor. It provides catalytic rate enhancements …
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Phosphorus physiology of the marine cyanobacterium Trichodesmium
… P metabolism in cyanobacteria include: inorganic phosphate (Pi) uptake, dissolved organic phosphorus (DOP) hydrolysis, and polyphosphate (polyP) biosynthesis and catabolism. These three pathways lay the foundation for the four chapters that make up the body of this thesis. Chapter two examines the …
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Effects of magnesium deficiency on urinary glycoproteins in the rat
… when compared to control animals. Increased phosphate binding by Fraction II from deficient animals was indicated. A slight rise in total glycoprotein excreted daily was shown in animals fed a magnesium deficient diet, but not until after the first week, when irreversible kidney damage is …
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Oil palm residue biodelignification by bacillus cereus ligninase from coptotermus curvignathus gut for handsheet production
… protein, glycosyltransferase, heme peroxidase, phosphate binding protein PstS, ABC transporter ATP-binding protein, nitrate reductase, and nitrite reductase. The delignification ability was tested on its chemical composition. Methods used followed technical association pulp and paper industry …
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Structure and regulation of yeast glycogen synthase
… regulated by the allosteric activator glucose-6-phosphate, which can overcome the inhibitory effects of phosphorylation. The effects of phosphorylation and glucose-6-phosphate on glycogen synthase are mediated by a cluster of six arginines located within a stretch of 12 amino acids near the …
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Phosphorus physiology of the marine cyanobacterium Trichodesmium
… P metabolism in cyanobacteria include: inorganic phosphate (Pi) uptake, dissolved organic phosphorus (DOP) hydrolysis, and polyphosphate (polyP) biosynthesis and catabolism. These three pathways lay the foundation for the four chapters that make up the body of this thesis. Chapter two examines the …
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Formylated dipyrromethane based phosphate derivatives receptors
… In Chapter 1, a brief introduction to anion binding chemistry is provided with a particular focus on phosphate anion recognition. The common defining feature of the receptors reviewed is the pyrrole ring. The new structures are often designed via modification of known macrocyclic molecules. …
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Formylated dipyrromethane based phosphate derivatives receptors
… In Chapter 1, a brief introduction to anion binding chemistry is provided with a particular focus on phosphate anion recognition. The common defining feature of the receptors reviewed is the pyrrole ring. The new structures are often designed via modification of known macrocyclic molecules. …
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Expression, sequencing, and characterization of mannitol-1- phosphate dehydrogenase genes from Aspergillus parasiticus and Escherichia coli
The genes coding for mannitol-1-phosphate dehydrogenases (mtlD) from Aspergillus parasiticus and Eschericia coli were cloned and sequenced. The two coding regions were highly homologous and the identity was 88.7% at the amino acid level and 81.6% at the nucleotide level. The two genes translate …
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The role of drosophila protein kinase doubletime in circadian period determination, morning and evening oscillators and tauopathy
… affected amino acids that are part of a proposed phosphate-binding domain. Additional mutations in the vicinity of the NLS shortened period, while mutations that were not close lengthened period. The shortening of period by some mutations suggests that they affect a TAU-like domain involved in a …
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Insights into the Development of Chemotherapeutics Targeting PFKFB Enzymes
… For both cases, citrate primarily occupied the binding site of Fructose-6-phosphate (F6P), competitively blocking F6P from binding. Additionally, a carboxy arm of citrate extended into the γ-phosphate binding site of ATP, sterically and electrostatically blocking the catalytic binding mode for …
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Understanding the Mechanism of Succinyl-CoA Synthetase Catalysis from Structural Studies
… use ATP/ADP in the catalytic reaction, the ATP-binding domain of Blastocystis hominis SCS (BhSCS) was co-crystallized with Mg2+-ADP. The 2.2 Å-resolution structure showed that this enzyme is ATP-specific due to the conformations of the backbone at Gln20ß, of the side chain of Gln20ß, and of the …
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