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University of Illinois at Urbana-Champaign
PaaG and PaaF: New Members of the Crotonase Superfamily
Abstract
dc:descriptionUsing the published operon structure of phenylacetate utilization, BLAST searches of public sequence databases, and metabolic information from a related compound, two pathways for phenylacetate metabolism were proposed. The genes encoding PaaF, PaaG, PaaH, PaaJ, and PaaI were cloned, and the proteins expressed, and purified. I showed by enzyme assays using purified proteins that beta-oxidation in phenylacetate metabolism proceeds via adipyl-CoA intermediates. I showed that PaaG catalyzes the isomerization of trans-Delta 3-hexenedioyl-CoA to trans-Delta2-hexenedioyl-CoA, uses an Asp as a general base, and the reaction proceeds without incorporation of solvent protons.
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Biochemistry
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2015
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Lies, Mark Andrew, III
- Contributors dc:contributor
-
- Gerlt, John A.
Subjects
dc:subject × 1Rights
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
- (MiAaPQ)AAI3086121
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/84793