Engineering a Purple copper(A) Site Into the Blue Copper Protein Azurin: Construction, Spectroscopy, and Metal Substitution Studies

Metal substitution studies of azurin-CuA have been performed using various metal ions (i.e., Hg2+, Ag+, Cu +, Cd+, Rh2+, Au+, Co 2+, and Ni2+). Metal binding was determined by electronic absorption and electrospray mass spectrometry. The results obtained from theses studies indicate that there are two factors that may influence binding in azurin-CuA: (1) the site tends to bind metal combinations which produce an overall +3 charge, and (2) the site binds soft thiophilic metals. Further characterization of the above derivatives will contribute to the understanding of the metal-binding affinity, rigidity, and other structural properties of purple copper centers.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Hay, Michael Todd

Contributors dc:contributor

• Lu, Yi

• Chemistry, Biochemistry

Language dc:language

eng

Identifier

(MiAaPQ)AAI9912257

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84423