{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/84423"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/84423","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Engineering a Purple copper(A) Site Into the Blue Copper Protein Azurin: Construction, Spectroscopy, and Metal Substitution Studies","abstract":"Metal substitution studies of azurin-CuA have been performed using various metal ions (i.e., Hg2+, Ag+, Cu +, Cd+, Rh2+, Au+, Co 2+, and Ni2+). Metal binding was determined by electronic absorption and electrospray mass spectrometry. The results obtained from theses studies indicate that there are two factors that may influence binding in azurin-CuA: (1) the site tends to bind metal combinations which produce an overall +3 charge, and (2) the site binds soft thiophilic metals. Further characterization of the above derivatives will contribute to the understanding of the metal-binding affinity, rigidity, and other structural properties of purple copper centers.","abstract_html":"Metal substitution studies of azurin-CuA have been performed using various metal ions (i.e., Hg2+, Ag+, Cu +, Cd+, Rh2+, Au+, Co 2+, and Ni2+). Metal binding was determined by electronic absorption and electrospray mass spectrometry. The results obtained from theses studies indicate that there are two factors that may influence binding in azurin-CuA: (1) the site tends to bind metal combinations which produce an overall +3 charge, and (2) the site binds soft thiophilic metals. Further characterization of the above derivatives will contribute to the understanding of the metal-binding affinity, rigidity, and other structural properties of purple copper centers.","abstract_has_math":false,"creators":["Hay, Michael Todd"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Lu, Yi"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:14:24Z","date_published":"2015-09-25T22:14:24Z","updated_at":"2026-07-22T22:26:23Z","subjects":["Chemistry, Biochemistry"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI9912257"],"render_values":[{"text":"(MiAaPQ)AAI9912257","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/84423","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Lu, Yi"]},{"key":"dc:creator","label":"Author","values":["Hay, Michael Todd"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:14:24Z","10000-01-01","1998"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Chemistry, Biochemistry"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/84423","(MiAaPQ)AAI9912257"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Metal substitution studies of azurin-CuA have been performed using various metal ions (i.e., Hg2+, Ag+, Cu +, Cd+, Rh2+, Au+, Co 2+, and Ni2+). Metal binding was determined by electronic absorption and electrospray mass spectrometry. The results obtained from theses studies indicate that there are two factors that may influence binding in azurin-CuA: (1) the site tends to bind metal combinations which produce an overall +3 charge, and (2) the site binds soft thiophilic metals. Further characterization of the above derivatives will contribute to the understanding of the metal-binding affinity, rigidity, and other structural properties of purple copper centers.","Made available in DSpace on 2015-09-25T22:14:24Z (GMT). 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Metal binding was determined by electronic absorption and electrospray mass spectrometry. The results obtained from theses studies indicate that there are two factors that may influence binding in azurin-CuA: (1) the site tends to bind metal combinations which produce an overall +3 charge, and (2) the site binds soft thiophilic metals. Further characterization of the above derivatives will contribute to the understanding of the metal-binding affinity, rigidity, and other structural properties of purple copper centers.","Made available in DSpace on 2015-09-25T22:14:24Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 9912257.pdf: 7171821 bytes, checksum: c4cf5fcc6cf8bd78ca065b53b0cecbee (MD5) Previous issue date: 1998","Embargo set by: Seth Robbins for item 85704 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","177 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1998."],"dc:identifier":["http://hdl.handle.net/2142/84423","(MiAaPQ)AAI9912257"],"dc:language":["eng"],"dc:subject":["Chemistry, Biochemistry"],"dc:title":["Engineering a Purple copper(A) Site Into the Blue Copper Protein Azurin: Construction, Spectroscopy, and Metal Substitution Studies"],"dc:type":["text"],"thesis:degree_discipline":["Chemistry"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:23Z"}