Progress in the Synthesis and Modification of Glass Surfaces Necessary to the Study of Single Molecule Protein Folding and Nanolithography

The focus of this research was to monitor the folding of a single protein molecule using sp-FRET. In order to accomplish this task, surface modification of a glass coverslip was necessary to prevent non-specific binding. Modification of the surface was accomplished by derivatizing the surface with PEG's and dextrans. Also, the surface was modified using a metal coated AFM tip, resulting in nanolithography of the surface which was observed using confocal microscopy.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Engebretson, David Alan

Contributors dc:contributor

• McDonald, J. Douglas

• Biophysics, General

Language dc:language

eng

Identifier

(MiAaPQ)AAI3202088

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84210