Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 49 for “"Non-specific binding"”.
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The Cocaine-Binding Aptamer: A Thermodynamic and Structural Overview of Specific and Non-Specific Binding Interactions
… wherein variances in the heat capacity of binding (Cp) among variants of the cocaine-binding aptamer were suggestive of two distinct binding mechanisms. Aptamer variants containing 6 base pairs in stem 1 are pre-folded and show little change in secondary structure with ligand binding. …
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The Design and Synthesis of Fluorescent Estrogen Receptor Reagents and Partitioning Measurements as a Model for Non-Specific Binding of Estrogens
Estrogens have been implicated in stimulating growth of certain forms of breast cancer which have heightened estrogen receptor levels. Fluorescence spectroscopy may allow for study of the estrogen receptor to determine the nature of the ligand-protein interactions as well as provide for a simple …
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Antibody Purification from Tobacco by Protein A Affinity Chromatography
… columns may be problematic due to the non-specific binding of native tobacco proteins (NTP). In this project, three different Protein A resins, ProSepvA High Capacity, ProSep-vA Ultra, and ProSep Ultra Plus, marketed by Millipore, were studied to provide valuable information for future …
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Progress in the Synthesis and Modification of Glass Surfaces Necessary to the Study of Single Molecule Protein Folding and Nanolithography
… of a glass coverslip was necessary to prevent non-specific binding. Modification of the surface was accomplished by derivatizing the surface with PEG's and dextrans. Also, the surface was modified using a metal coated AFM tip, resulting in nanolithography of the surface which was observed using …
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Examination of UV-Cross-Linkable Di-Block Copolymer Strategy for Functionalized Reaction Surface on Microelectrode Arrays
… and relatively inert with respect to its non-specific binding with receptors. This work makes progress towards this goal by first exploring the Pd(0) chemistry on the array, identifying the incompatibility of palladium chemistry with the agarose coating that was being used on the surface …
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Smart, biocompatible semi-conductor nanocrystal constructs designed for in-vitro imaging applications
… these shortcomings. First, the issues of non-specific binding and reactivity are alleviated by exploring a new method to conjugate molecules onto the QD surface. The improvements that were made enabled a collaborator situated across the country to conjugate biomolecules in a one-step …
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Proteomic Profiling of Pro and Active Matrix Metalloproteinases using Tandem Mass Spectrometry. Optimization of Affinity Chromatography and nHPLC-MALDI-MS/MS for Proteomic discrimination of Matrix Metalloproteinases in pre-clinical Cancer Model.
… different roles. Understanding and assigning specific role to each of 24 members of these endoproteinases is impeded because of lack of specific and efficient detection methods in biological samples. Moreover, MMP-based anti-cancer drug development has also been challenged because, currently, …
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A model for sigma factor competition in bacterial cells
… pattern of expressed genes. In response to specific environmental stresses the cell activates several stress-specific molecules such as sigma factors. They reversibly bind the RNA polymerase to form the so-called holoenzyme and direct it towards the appropriate stress response genes. In …
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Development of chemical inducers of dimerization for screening competitive histone deactelyase inhibitors
… Currently, most of the known HDAC inhibitors are non-selective, which causes non-specific binding to the active sites of all HDAC isoforms, including those not involved in cancer. To date, screening for HDAC inhibitors has been done using an in vitro fluorescence-screening assay, which is unable …
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Detection of glial interleukin-6 release: Development of a novel interleukin-6 Elisa
… were attempted including matrix effects, non-specific binding, and coating buffer pH differences. In comparison to the ELISA, the LOD for IL-6 using the 7TD1 bioassay was 2--5 pg/mL. Therefore the 7TD1 bioassay was implemented for the experiments described in this study. IL-6 is a cytokine …
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Switchable biological surfaces
… Fluorescence images indicated that less binding took place between the neutravidin and the biotinylated peptide under a negative potential due to decreased image intensity. Control experiments were also carried out using non-biotinylated peptides to show there was minimal non-specific …
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Single molecule investigation of transcription activator-like effector search dynamics
… to the study the TALE search process along specific and non-specific DNA templates. Our work provides a molecular-level picture of the dynamics of TALE-DNA interactions, and our results have revealed an apparently unique search mechanism for DNA binding proteins. We directly observe TALEs …
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DEVELOPMENT OF SURFACE BASED PLATFORMS FOR BIOMOLECULAR RECOGNITION AND PROTEASE ASSAYS
… substrates in our laboratory, capable of binding with the surfaces of study. In addition, we present methods to on how to address non-specific binding issues inherited by the nature of these assays. Commercially available recombinant proteases have been utilized throughout these studies. …
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Sensitive Detection of Foodborne E. coli O157:H7 by Dendrimer-aptamer Modified Microchannels - with RCA Signal Intensifications
… siloxane (PDMS) microchannels to avoid non-specific binding and to provide more binding sites to subsequently conjugate capturing aptamers as recognition elements. Surface modifications by the PAMAM dendrimers and the aptamers are characterized. To further enhance detection sensitivity, …
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Detection and enrichment of cytochrome P450s using bespoke affinity chromatography and proteomic techniques. Development of chemical immobilisation and novel affinity chromatography methods, with subsequent proteomic analysis, for the characterisation of cytochrome P450s important in cancer research.
… activate cancer therapeutics. Identifying specific P450s that are highly up-regulated at the tumour site could be used to predict drug response and formulate targeted cancer therapy to help diminish systemic side-effects. Methods: Previous enrichment strategies have been unable to isolate …
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Site-specific immobilization of tetrazine-modified green fluorescent protein on trans-cyclooctene surfaces
… biomaterials, tissue engineering and biosensors. Non-covalent protein adsorption (physisorption), the easiest of all protein-attachment approaches, suffers from several drawbacks and was rejected for this work. Although the covalent attachment (chemisorption) of peptides and proteins to solid …
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Development of highly specific monoclonal antibodies to Listeria sp. and Listeria monocytogenes
… onto agarose. In conclusion, the production of specific monoclonal antibodies against attenuated Listeria grown at 37°C was shown to be feasible. Two monoclonal lines were produced. One line was unstable, but detected 9 out of 10 of the Listeria strains. None of the positive cell wells in all …
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Engineering semiconductor quantum dots for quantitative imaging of cell motility and invasion
… chemical moieties were used to quantify non-specific binding to different extracellular matrix (ECM) proteins. QDs coated with poly-maleic anhydride (PMA), which had been modified to present alkane, alkene, alkyne, PEG and carboxylic acid, carboxylic acid, and solely PEG, were incubated …
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Genetic and behavioral discrimination of dopamine 1 and 5 receptors in hippocampal dependent memory consolidation
… in an analogous fashion. Additionally, the specific expressional pattern of each receptor lacks clarity due to non-specific binding by molecular probes. Moreover, D1 and D5 pharmacological and global KO studies cannot and have not functionally delineated D1Rs from D5Rs and global KOs of the …
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