Design of the First Analytical Platform for Automated Top Down Proteomics With quadrupole/Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

The enclosed writing details technology development leading to the first high-throughput proteome analysis of Methanosarcina acetivorans utilizing a custom FT mass spectrometer. It outlines a two dimensional separations platform that utilizes a 96 well plate nanospray robot to facilitate automated analysis on the mass spectrometer. This thesis also highlights instrument advancements required to improve throughput for observation of protein intact masses and methods used to obtain robust fragmentation. Included within the automation platform is software developed for efficiently managing intact protein and MS/MS data. Also, a novel fragmentation method has been developed to extend dynamic range for fragmentation of intact proteins as large as 60 kDa. The enclosed work is the foundation for the first fully automated platform for intact protein analysis by tandem MS. With the identification of hundreds of proteins from a single run, the high-throughput Top Down proteome analysis of intact proteins is now a reality.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Patrie, Steven M.

Contributors dc:contributor

• Kelleher, Neil L.

• Biology, Molecular

Language dc:language

eng

Identifier

(MiAaPQ)AAI3153395

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84159