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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Intact Proteins"”.
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Bioinformatics of High Throughput Proteomics Using Tandem Mass Spectrometry of Intact Proteins
… to the problem of identifying and characterizing proteins with a DNA-predicted sequence. It would be desirable to move top down mass spectrometry in to the 'omic' sciences by developing a high throughput form that could be used to simultaneously identify and characterize thousands of proteins. …
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Direct Analysis of Intact Proteins From Microorganisms Using Top Down Mass Spectrometry
… this top down proteomics platform to study proteins from Methanococcus jannaschii and Saccharomyces cerevisiae. From the whole cell lysate of yeast, ∼120 ion species were successfully identified with 100% sequence coverage, and we also detected several post-translational modifications, …
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A Comparison of Assay Techniques for Determining the Availability to the Chick of Amino Acids in Intact Proteins
Made available in DSpace on 2014-12-09T14:28:57Z (GMT). No. of bitstreams: 1 6706646.pdf: 3498555 bytes, checksum: c311d68dc1156fabf372d2b62482de3d (MD5) Previous issue date: 1966
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Factors Influencing the Growth of Chicks Fed Crystalline Amino Acid Dietswith Special Reference to a Growth Stimulating Factor in Intact Proteins
Made available in DSpace on 2014-12-05T17:47:42Z (GMT). No. of bitstreams: 1 6003922.pdf: 4419794 bytes, checksum: 887f0089405fe8c9b6ec4462f52b5a0c (MD5) Previous issue date: 1960
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The Utilization of The Amino Acids in Intact Proteins by The Growing Chick, With Special Reference to The Effect of Heating Upon Amino Acid Utilization, as Measured by Amino Acid Supplementation, Biological (Chick) Assay of Amino Acids and Free-Amino Acid Patterns of Blood Plasma
Made available in DSpace on 2014-12-09T14:28:46Z (GMT). No. of bitstreams: 1 6406156.pdf: 6391908 bytes, checksum: a06d1d53203db22de00eefe1345754d5 (MD5) Previous issue date: 1963
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Design of the First Analytical Platform for Automated Top Down Proteomics With quadrupole/Fourier Transform Ion Cyclotron Resonance Mass Spectrometry
… to improve throughput for observation of protein intact masses and methods used to obtain robust fragmentation. Included within the automation platform is software developed for efficiently managing intact protein and MS/MS data. Also, a novel fragmentation method has been developed to extend …
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Multidimensional liquid chromatographic separations for proteomics
… employed to reduce this complexity, both at an intact protein and digested peptide level. For complex lysates containing many thousands of proteins, orthogonal (mutually independent), multidimensional separation methods must be employed to provide sufficient resolution to characterize the …
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Superantigens as vaccine delivery vehicles for the generation of cellular immune responses
… like conventional antigens but instead bind as intact proteins to MHC class II molecules expressed on the surface of professional antigen presenting cells. On the hypothesis that the unique properties of superantigens may serve useful for vaccine delivery, several bacterial superantigens were …
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Geochemical controls on the distribution and composition of biogenic and sedimentary carbon
… Mn oxides is primarily proteinaceous, including intact proteins involved in Mn oxidation and likely oxide nucleation and aggregation. Pelagic sediments from 16 sites underlying the South Pacific and North Atlantic gyres and spanning a gradient of sediment age and redox state were analyzed in …
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Improved Dynamic Range, Quantitation, and Characterization of Histone H4 Post-Translational Modifications: A Top Down Mass Spectrometric Approach
"Intimately associated with DNA, histone proteins serve as both a structural scaffold for DNA packaging into the nucleus and an epigenetic means for the regulation of gene expression. One such histone-based mechanism for transcriptional regulation is post-translational modification (PTM) of …
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Geochemical controls on the distribution and composition of biogenic and sedimentary carbon
… Mn oxides is primarily proteinaceous, including intact proteins involved in Mn oxidation and likely oxide nucleation and aggregation. Pelagic sediments from 16 sites underlying the South Pacific and North Atlantic gyres and spanning a gradient of sediment age and redox state were analyzed in …
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Characterization of Microorganisms of Interest to Homeland Security and Public Health Utilizing Liquid Chromatography/Mass Spectrometry
… differences in the LC/MS data. Reproducible intact protein biomarkers were discovered based on their unique mass, retention time and relative intensity. These markers were implemented to differentiate closely related strain types, (e.g. two distinct isolates of E. coli O157:H7) and to …
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Development and application of OmpT-based middle down proteomics
… post-translational modifications (PTMs) on proteins, and ultimately, quantification of proteins or their PTM levels from given biological samples, mostly using mass spectrometry. Modern mass spectrometry-based proteomic analyses primarily fall into two categories in terms of general …
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Quantification and alleviation of the antinutritional effects of phytate on poultry
… resulting in heightened presence of intact proteins in the tract, which instigates an increase in secretion of HCl and pepsin. More bicarbonate ions are released in response to this increased acidity, causing an increase in intestinal pH and resulting reduction in solubility of …