Back to results
University of Illinois at Urbana-Champaign
In Vitro Selection and Characterization of Transition Metal -Dependent DNAzymes and RNAzymes
Abstract
dc:description"These DNAzymes are dependent on Co2+ for cleavage activity. By exploiting the electronic properties of Co2+, we have used UV-Visible spectroscopy to obtain a better understanding of the metal binding properties of the selected DNAzyme systems. Through analysis of the spectroscopic and metal binding properties of these systems, in conjunction with the activity properties, a better understanding of how metals are binding in these systems has been developed. A previously selected DNAzyme system, the ""8-17"" motif, and a naturally-occurring RNAzyme system, the hammerhead motif, were also further examined in terms of the diversity of conditions under which RNA cleavage activity is supported."
Degree
thesis:*- Name thesis:degree_name
- Ph.D.
- Level thesis:degree_level
- Dissertation
- Discipline thesis:degree_discipline
- Chemistry
- Grantor
- University of Illinois at Urbana-Champaign
- Year dc:date
- 2015
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Bruesehoff, Peter John
- Contributors dc:contributor
-
- Lu, Yi
Subjects
dc:subject × 1Rights
- Language dc:language
- eng
Identifiers
dc:identifier.*- Identifier
- (MiAaPQ)AAI3101806
- OAI identifier oai:identifier
- oai:www.ideals.illinois.edu:2142/84113