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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"DNAzymes"”.
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Investigation of Metal-Dependence in DNAzymes and Applications of DNAzymes and Aptamers for Diagnostics
… in diagnostics and sensing were examined using DNAzymes and aptamers. Aptamers are nucleic acids which bind a specific chemical or biological moeity. By labeling DNAzymes and aptamers with colored gold nanoparticles, our laboratory had previously constructed colorimetric sensors for analytes …
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Cellular metal ion sensing using DNAzymes
… metal ions or to meet new desired criteria. DNAzymes, DNA sequences with catalytic activity, have recently emerged as an alternative class of sensors for metal ions. As selection of DNAzymes for different metal ions is carried out through a combinatorial process, new sequences with desired …
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The Development of Gene Therapy by way of DNAzymes, and a Computational Tool that Predicts Efficient DNAzymes.
… target the androgen receptor (AR) is required. DNAzymes, single stranded antisense molecules constructed of DNA, have promise as a novel therapeutic strategy to down-regulate AR expression and therefore inhibit tumour growth. An assay pipeline of DNAzyme cleavage reactions, targeting the AR and …
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DNAzymes for sequence-dependent peptide lysine acylation
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2025-10-20 without embargo terms
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DNAzymes for amine and peptide lysine acylation
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2023-12-01
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In vitro selection of monovalent metal ion-dependent DNAzymes
… reactions, these DNA sequences are called DNAzymes, or DNA enzymes. Like with protein enzymes, the sequence of a DNAzyme dictates the folding of DNA into unique tertiary structure which ultimately programs its catalytic function. The folding of DNA is a product of a combination of …
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Fluorescent and Colorimetric Biosensors Based on DNAzymes and DNA Aptamers
… nucleic acids, a large number of catalytic DNA (DNAzymes) and aptamers have been isolated, many of which possess high analyte specificity. As a result, these functional nucleic acids can be used for sensing and diagnostic applications. However, the number of successful sensors made from these …
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In Vitro Selection and Metal Specificity of Transition Metal-Dependent DNAzymes
… of two families of Co2+-dependent DNAzymes further demonstrates the interplay between DNAzyme structure and metal specificity. The clone 11 and 18 DNAzymes were selected for study because the primary sequences differ by only four bases, producing vast differences in Co2+ …
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DNAzymes as intracellular sensors for metal ion imaging and their structural characterization
Embargo set by: Seth Robbins for item 91427 Lift date: 2018-03-02T21:07:27Z Reason: Author requested closed access (OA after 2yrs) in Vireo ETD system
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In Vitro Selection and Characterization of Transition Metal -Dependent DNAzymes and RNAzymes
"These DNAzymes are dependent on Co2+ for cleavage activity. By exploiting the electronic properties of Co2+, we have used UV-Visible spectroscopy to obtain a better understanding of the metal binding properties of the selected DNAzyme systems. Through analysis of the spectroscopic and metal …
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Methods and applications of using DNA-cleaving DNAzymes to cleave double-stranded DNA
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01
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In Vitro Selection and Characterization of Highly Selective Metal -Dependent DNAzymes With Potential Biosensor Applications
… the secondary structures of the resulting DNAzymes with the structure of the 8-17-like DNAzymes, which were selected through three independent experiments in the presence of: (1) Zn2+, (2) Mg2+, and (3) histidine plus Mg2+. The DNAzymes obtained from this experiment did not appear to …
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The in vitro selection and biochemical characterization of metalloDNAzymes
DNAzymes are strands of catalytic DNA. First discovered in 1994, they have proved themselves capable of catalyzing many different types of reactions with significant rate enhancements. Because they often require divalent metal-ion cofactors, DNAzymes have readily been developed into metal-ion …
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In vitro selection and characterization of mono-, di-, and trivalent metal-dependent DNAzymes and their sensing applications
… high affinity and selectivity. Catalytic DNA or DNAzymes, another class of functional nucleic acids, were first isolated from pools of random DNA in 1994 using the in vitro selection procedure to catalyze the cleavage of a phosphodiester bond. Since the discovery of the first aptamer and DNAzyme, …
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In vitro selection of DNAzymes selective for monovalent metal ions and their development as sensors in living cells and organisms
… the catalytic function (deoxyribozymes or DNAzymes) of DNAs have been explored since 1990s, which attract widespread interest. DNAzymes, fully biocompatible but not naturally identified, commonly use metal ions as cofactors for catalysis, and some of them show exceptional selectivity for …
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G-Triple Chemiluminescent DNAZyme Based Biosensor for the Detection of Calcium(II) Ions in Solution and the Biological Functionality of Kinase Enzymes and the Rationale for their Employment as Promising Drug Targets, Substrate Specific Inhibition of P38alpha
… as recognition elements in bioanalytical assays. DNAzymes are functionally active, synthetic single-stranded DNA molecules that are capable of catalyzing biological reactions similarly to proteins or RNAzymes. The majority of DNAzymes are synthesized by an in vitro selection method known as …
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Novel Techniques to Target Androgen Signalling in Prostate Cancer
… proteins driving PCa growth and to design DNAzymes (single stranded antisense molecules constructed of DNA) to down-regulate androgen receptor (AR) expression. We identified a DNAzyme, AM-3 that can degrade AR RNA and inhibit the proliferation of the LNCaP PCa cell line. A program was also …
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I. In vitro selection of aptamers for perchlorate and melamine II. Towards crystallization of DNAzymes III. A highly selective lead sensor based on a classic lead DNAzyme
… After the initial discovery of aptamers and DNAzymes, many more functional nucleic acids have been isolated for binding a wide variety of ligands and catalyzing a number of chemical transformations. The unique properties of nucleic acids have made functional nucleic acids suitable for wide …
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Applications of functional nucleic acids in imaging, sensing and drug delivery and investigations of DNAzyme-metal interactions
… including nucleic acids catalysts (ribozymes and DNAzymes) and ligands (aptamers), have been discovered in nature or isolated in a laboratory through a process called in vitro selection. They are nucleic acids with functions similar to protein enzymes or antibodies. They have been developed into …
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Exploring the Structural and Functional Characteristics of a Catalytic DNA
Finally, the use of DNAzymes in biosensing applications was studied for Pb2+ detection. The molecular beacon DNAzyme biosensor was shown to be capable of detecting Pb2+ in soft to moderately hard water sources when used in conjunction with a handheld fluorometer. Detection limits under these …
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