Novel Mass Spectrometric and Sampling Strategies for Investigating Neuropeptide Processing and Distribution

Direct mass spectrometric analysis, combined with knowledge of expressed genes, often provides enough information to identify peptides, to determine new processing pathways, and to assess genetic variations. In some instances, however, alternative sampling approaches such as microseparation with MALDI MS detection are necessary to pre-concentrate and partially purify neuropeptides and to reduce ionization suppression effects. Here we describe the use of capillary electrophoresis (CE) coupled with complementary detection schemes MALDI MS and radionuclide detection for the determination of trace expression of neuropeptides in the cerebral ganglion A cluster.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemistry

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Kruse, Rebecca Anne

Contributors dc:contributor

• Sweedler, Jonathan V.

• Biology, Neuroscience

Language dc:language

eng

Identifier

(MiAaPQ)AAI3070360

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/84082