{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/84082"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/84082","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Novel Mass Spectrometric and Sampling Strategies for Investigating Neuropeptide Processing and Distribution","abstract":"Direct mass spectrometric analysis, combined with knowledge of expressed genes, often provides enough information to identify peptides, to determine new processing pathways, and to assess genetic variations. In some instances, however, alternative sampling approaches such as microseparation with MALDI MS detection are necessary to pre-concentrate and partially purify neuropeptides and to reduce ionization suppression effects. Here we describe the use of capillary electrophoresis (CE) coupled with complementary detection schemes MALDI MS and radionuclide detection for the determination of trace expression of neuropeptides in the cerebral ganglion A cluster.","abstract_html":"Direct mass spectrometric analysis, combined with knowledge of expressed genes, often provides enough information to identify peptides, to determine new processing pathways, and to assess genetic variations. In some instances, however, alternative sampling approaches such as microseparation with MALDI MS detection are necessary to pre-concentrate and partially purify neuropeptides and to reduce ionization suppression effects. Here we describe the use of capillary electrophoresis (CE) coupled with complementary detection schemes MALDI MS and radionuclide detection for the determination of trace expression of neuropeptides in the cerebral ganglion A cluster.","abstract_has_math":false,"creators":["Kruse, Rebecca Anne"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Chemistry","degree_department":null,"school":null,"contributors":["Sweedler, Jonathan V."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T22:12:47Z","date_published":"2015-09-25T22:12:47Z","updated_at":"2026-07-22T22:26:22Z","subjects":["Biology, Neuroscience"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3070360"],"render_values":[{"text":"(MiAaPQ)AAI3070360","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/84082","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Sweedler, Jonathan V."]},{"key":"dc:creator","label":"Author","values":["Kruse, Rebecca Anne"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T22:12:47Z","10000-01-01","2002"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Chemistry"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Neuroscience"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/84082","(MiAaPQ)AAI3070360"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Direct mass spectrometric analysis, combined with knowledge of expressed genes, often provides enough information to identify peptides, to determine new processing pathways, and to assess genetic variations. In some instances, however, alternative sampling approaches such as microseparation with MALDI MS detection are necessary to pre-concentrate and partially purify neuropeptides and to reduce ionization suppression effects. Here we describe the use of capillary electrophoresis (CE) coupled with complementary detection schemes MALDI MS and radionuclide detection for the determination of trace expression of neuropeptides in the cerebral ganglion A cluster.","Made available in DSpace on 2015-09-25T22:12:47Z (GMT). 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In some instances, however, alternative sampling approaches such as microseparation with MALDI MS detection are necessary to pre-concentrate and partially purify neuropeptides and to reduce ionization suppression effects. Here we describe the use of capillary electrophoresis (CE) coupled with complementary detection schemes MALDI MS and radionuclide detection for the determination of trace expression of neuropeptides in the cerebral ganglion A cluster.","Made available in DSpace on 2015-09-25T22:12:47Z (GMT). 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