Biochemical and Molecular Approaches to Characterizing the Myrosinase Enzyme System in Horseradish (Armoracia Rusticana)

A full length cDNA (ArM1) encoding myrosinase was cloned from horseradish root by RACE-PCR. ArM1 has an open reading frame of 1,614 nucleotides with a deduced protein of 538 amino acid and molecular mass of 61.6 kDa. Heterologous expression of ArM1 in Spodoptera frugiperda insect cells resulted in an immune active myrosinase protein product and gave rise to a band of approximately 66 kDa in western blot analysis. ArM1 has the highest overall amino acid identity (72%) with Arabidopsis thaliana myrosinase TGG2. Phylogenetic analysis shows that ArM1 gene does not cluster with any of the currently known myrosinase subfamily and it may represent another myrosinase subfamily in root tissue.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Natural Resrouces and Environmental Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Li, Xian

Contributors dc:contributor

• Kushad, Mosbah M.

• Agriculture, Food Science and Technology

Language dc:language

eng

Identifier

(MiAaPQ)AAI3160916

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/83099