Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 165 for “"Open Reading Frame"”.
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Understanding the Role of the Hypervariable Region in the Open Reading Frame 1 of the Hepatitis E virus in Viral Replication
… identified a hypervariable region (HVR) in the open reading frame 1 (ORF1) of HEV. The objectives of this dissertation were to utilize chicken and swine model systems to study the role of HVR in HEV infection in vivo, to determine the effects of HVR on replication of HEV in vitro, and to analyze …
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Genome-wide sequencing and an open reading frame analysis of DDT susceptible (91-C) and resistant (91-R) Drosophila melanogaster laboratory populations
… lines to identify structural changes within the open reading frames (ORFs) to identify putative resistance loci across the entire genome. Allele fixation revealed a higher number of novel alleles going to fixation for the DDT resistant 91-R fly line under intense selective pressure as compared to …
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Characterisation of the virulence-related, outer-membrane proteins of B. pertussis
… sequence led to the identification of a larger open reading frame which had not been previously recognised. The cosmid did not contain the entire open reading frame and a further 200 bp was obtained using an adapter-linked single specific primer-PCR (SSP-PCR). An open reading frame was predicted …
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Genetic studies on the region downstream of the unc operon of Thiobacillus ferroxidans
… and sequenced in both directions. One partial open reading frame (ORFl) and two complete open reading frames (ORF2 and ORF3) were found. On the basis of high homology to previously published sequences, ORFI was found to be the C-terminus of the T ferrooxidans glmU gene encoding the enzyme …
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Characterization of the genes and gene products of the acetate-activating enzymes and a novel iron-sulfur flavoprotein from Methanosarcina thermophila strain TM-1
… The two genes were sequenced, along with a third Open Reading Frame (designated <i>orfY</i>). The <i>orfY</i> gene appears to encode a novel protein whose physiological function has yet to be determined.
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Isolation and characterisation of an Hsp90 homologue from the resurrection plant Xerophyta viscosa
… of genes. The putative gene contained an ORF (Open Reading frame) potentially coding for an 812 amino acid protein with a calculated size of 92.83 kDa. It shares 85% homology with other Hsp90s from plants and it contains several characteristic features of these proteins. Additionally, it …
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Regulation of the speC gene encoding ornithine decarboxylase in Escherichia coli by putrescine, spermidine and cAMP
… a putative CRP binding site and a downstream open reading frame (ORF2) present on the strand complementary to speC, was fused to lacZ to create a transcriptional fusion, pCC2L. Analysis of E. coli CB806/pCC2L revealed that there was no detectable β8- galactosidase activity from the ORF2-lacZ …
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Búsqueda de mutaciones en el gen foxo4 en pacientes con falla ovárica prematura no-sindrómica
… exclude that FOXO4 dysfunctions, originated from open reading frame or promoter sequence variations, might be associated with the pathogenesis of the disease in other ethnical groups.
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Biochemical and Molecular Approaches to Characterizing the Myrosinase Enzyme System in Horseradish (Armoracia Rusticana)
… from horseradish root by RACE-PCR. ArM1 has an open reading frame of 1,614 nucleotides with a deduced protein of 538 amino acid and molecular mass of 61.6 kDa. Heterologous expression of ArM1 in Spodoptera frugiperda insect cells resulted in an immune active myrosinase protein product and gave …
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An investigation into the specific function of the vaccinia virus :13.8 kDa protein encoded by the N1L gene
… has characteristic variable termini encoding open reading frames that are not essential for virus replication in cell culture. One such open reading frame, N1L situated at the left terminal region of the neurovirulent Western Reserve (WR) vaccinia virus strain, encodes a protein 13.8 kDa in …
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An investigation into the specific function of the vaccinia virus 13.8 kDa protein encoded by the N1
… has characteristic variable termini encoding open reading frames that are not essential for virus replication in cell culture. One such open reading frame, N1L situated at the left terminal region of the neurovirulent Western Reserve (WR) vaccinia virus strain, encodes a protein 13.8 kDa in …
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Identification of a Low Molecular Weight Protein Tyrosine Phosphatase and Its Potential Physiological Substrates in Synechocystis sp. PCC 6803
The predicted protein product of open reading frame slr0328 from Synechocystis sp. PCC 6803, SynPTP, possesses significant amino acid sequence similarity with known low molecular weight protein tyrosine phosphatases (PTPs). To determine the gross functional properties of this hypothetical protein, …
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The nucleotide sequence of the 3' terminus of soybean mosaic virus
… an insert of 1443 nucleotides and had an open reading frame of 1119 nucleotides terminating 224 nucleotides from the 3' terminal poly(A) tract. The coat protein cistron identified by a glutamine:serine dipeptide cleavage site 792 nucleotides upstream from the termination sequence could …
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Identification and Characterization of Aer, an Energy Sensor in Escherichia Coli
… A computerized search of GenBank revealed an open reading frame at 69.1 min on the E.co//chromosome that had a C-terminal fragment with 96.7% identity to the highly conserved domain of Tsr. The open reading frame was renamed aer for aerotaxis and energy responses. When the aer gene was …
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PCR-based cloning, characterization, and stress-induced expression of chitinase genes in Kentucky bluegrass (Poa pratensis L.)
… bluegrass genome. Chi1 and chi2 each contain an open reading frame with no introns, encoding polypeptides of 340 and 320 amino acids, respectively. Both CHI1 and CHI2, the predicted proteins encoded by chi7 and chi2, are class I chitinases and share 94% amino acid identity. CHI1 has a short …
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Molecular biology of segment 8 of the influenza A virus genome
… are encoded in the positive sense. A large open reading frame exists on segment 8 of the influenza A genome in the opposite sense to, yet overlapping with, the established proteins expressed from that segment; this hypothetically encodes a protein named Negative Strand Protein or NSP. This …
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Esi47, a stress-inducible protein kinase gene from Lophopyrum elongatum is involved in plant hormone signaling
… region of its mRNA a small upstream open reading frame that has been demonstrated to mediate the ABA regulation of Esi47 expression in an independent study. Three Arabidopsis homologs of Esi47 were identified and different members of this clade of genes showed differential patterns of …
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The Mycobacterium smegmatis "Proteome" : effects of growth phase on total protein synthesis and on the response to heat shock
… named Hspl7-2) showed significant homology to open reading frame 28 of M tuberculosis cosmid MTCY01B2. This is the first time a functional characteristic has been assigned to this open reading frame, and it remains to be seen if Hspl 7-2 represents a new family of heat shock proteins. Synthesis …
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Impatiens Necrotic Spot Virus Resistance in Transgenic Impatiens walleriana and Lycopersicon esculentum
… markers, and the antisense sequence of open reading frame of INSV nucleocapsid protein (N). The N gene is expected to confer INSV resistance by RNA interference or gene silencing. The presence of transgenes was confirmed by PCR. Transgenic Impatiens was selfed for two generations. …
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Characterization of the <i>glpD</i> and <i>glpEGR</i> operons of <i>Escherichia coli</i> k-12
… <i>GlpR</i> and <i>glpD</i> are separated by two open reading frames, designated <i>glpE</i> and <i>glpG</i>, encoding proteins of unknown function. The <i>glpD</i>-encoded aerobic sn-glycerol 3-phosphate dehydrogenase is a cytosplasmic membrane-associated respiratory enzyme. The nucleotide …
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