Introgression of the Genes for Sesquiterpene Carboxylic Acid Synthesis From a Wild Tomato, Lycopersicon Hirsutum, Into the Cultivated Tomato for Increased Host -Plant Resistance to Insect Pests

F2 and backcross progeny were assayed for the presence of polymorphic molecular markers using the Amplified Fragment Length Polymorphism (AFLP) protocol. Progeny of each generation were separated into high and low classes for SCA levels and the DNA combined of individual plants within the phenotypic classes. Bulk segregant analysis was used to identify polymorphic AFLP markers present in LA 1777 and absent in 'Chico III' and LA 1033. Comparisons of these polymorphic AFLP markers between high-SCA bulks and the zero-SCA plants ('Chico III' and LA 1033) revealed 10 AFLP markers associated with presence of SCA. Comparisons of the high-SCA bulks with a set of low-SCA bulks revealed one marker associated with high levels of SCA synthesis. Comparisons of the markers with a TA517 line that produces sesquiterpene precursors to SCA revealed three markers putatively associated with synthesis of the olefin to SCA and six putatively associated with modifications to SCA.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Natural Resrouces and Environmental Sciences

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Frelichowski, James Edward, Jr

Contributors dc:contributor

• Juvik, John A.

• Agriculture, Plant Pathology

Language dc:language

eng

Identifier

(MiAaPQ)AAI3044090

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/83081