{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/83081"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/83081","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"Introgression of the Genes for Sesquiterpene Carboxylic Acid Synthesis From a Wild Tomato, Lycopersicon Hirsutum, Into the Cultivated Tomato for Increased Host -Plant Resistance to Insect Pests","abstract":"F2 and backcross progeny were assayed for the presence of polymorphic molecular markers using the Amplified Fragment Length Polymorphism (AFLP) protocol. Progeny of each generation were separated into high and low classes for SCA levels and the DNA combined of individual plants within the phenotypic classes. Bulk segregant analysis was used to identify polymorphic AFLP markers present in LA 1777 and absent in 'Chico III' and LA 1033. Comparisons of these polymorphic AFLP markers between high-SCA bulks and the zero-SCA plants ('Chico III' and LA 1033) revealed 10 AFLP markers associated with presence of SCA. Comparisons of the high-SCA bulks with a set of low-SCA bulks revealed one marker associated with high levels of SCA synthesis. Comparisons of the markers with a TA517 line that produces sesquiterpene precursors to SCA revealed three markers putatively associated with synthesis of the olefin to SCA and six putatively associated with modifications to SCA.","abstract_html":"F2 and backcross progeny were assayed for the presence of polymorphic molecular markers using the Amplified Fragment Length Polymorphism (AFLP) protocol. Progeny of each generation were separated into high and low classes for SCA levels and the DNA combined of individual plants within the phenotypic classes. Bulk segregant analysis was used to identify polymorphic AFLP markers present in LA 1777 and absent in 'Chico III' and LA 1033. Comparisons of these polymorphic AFLP markers between high-SCA bulks and the zero-SCA plants ('Chico III' and LA 1033) revealed 10 AFLP markers associated with presence of SCA. Comparisons of the high-SCA bulks with a set of low-SCA bulks revealed one marker associated with high levels of SCA synthesis. Comparisons of the markers with a TA517 line that produces sesquiterpene precursors to SCA revealed three markers putatively associated with synthesis of the olefin to SCA and six putatively associated with modifications to SCA.","abstract_has_math":false,"creators":["Frelichowski, James Edward, Jr"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Natural Resrouces and Environmental Sciences","degree_department":null,"school":null,"contributors":["Juvik, John A."],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T21:00:19Z","date_published":"2015-09-25T21:00:19Z","updated_at":"2026-07-22T22:26:20Z","subjects":["Agriculture, Plant Pathology"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3044090"],"render_values":[{"text":"(MiAaPQ)AAI3044090","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/83081","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Juvik, John A."]},{"key":"dc:creator","label":"Author","values":["Frelichowski, James Edward, Jr"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T21:00:19Z","10000-01-01","2002"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Natural Resrouces and Environmental Sciences"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Agriculture, Plant Pathology"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/83081","(MiAaPQ)AAI3044090"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["F2 and backcross progeny were assayed for the presence of polymorphic molecular markers using the Amplified Fragment Length Polymorphism (AFLP) protocol. 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Progeny of each generation were separated into high and low classes for SCA levels and the DNA combined of individual plants within the phenotypic classes. Bulk segregant analysis was used to identify polymorphic AFLP markers present in LA 1777 and absent in 'Chico III' and LA 1033. Comparisons of these polymorphic AFLP markers between high-SCA bulks and the zero-SCA plants ('Chico III' and LA 1033) revealed 10 AFLP markers associated with presence of SCA. Comparisons of the high-SCA bulks with a set of low-SCA bulks revealed one marker associated with high levels of SCA synthesis. Comparisons of the markers with a TA517 line that produces sesquiterpene precursors to SCA revealed three markers putatively associated with synthesis of the olefin to SCA and six putatively associated with modifications to SCA.","Made available in DSpace on 2015-09-25T21:00:19Z (GMT). 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