In Vitro Selection and Characterization of Catalytic DNA in Model Biological Systems

This research also details the first attempt to obtain useful information from heterogeneous DNA sequencing data. A sample of the selection population from each of the twelve rounds of in vitro selection was heterogeneously sequenced without any prior separation or purification. The observed concurrence of qualitative changes in the heterogeneous sequencing chromatograms with observed changes in the catalytic activity of the population is evidence of the ability of heterogeneous DNA sequencing to successfully monitor the evolution of DNA populations during in vitro selection. The ability to successfully analyze heterogeneous DNA samples containing multiple DNA sequences using a single sequencing experiment may play an important role in driving the development of new DNA sequencing technologies and shaping the future of genomic research.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Materials Science and Engineering

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Slimmer, Scott Collins

Contributors dc:contributor

• Gerard Wong

• Biology, Bioinformatics

Language dc:language

eng

Identifier

(MiAaPQ)AAI3363093

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/82850