{"id":{"repo_id":"uiuc","oai_identifier":"oai:www.ideals.illinois.edu:2142/82850"},"canonical_url":"https://search.dev.ndltd.org/etd/uiuc/oai:www.ideals.illinois.edu:2142/82850","repository":{"repo_id":"uiuc","name":"University of Illinois - Urbana-Champaign","base_url":"https://www.ideals.illinois.edu/oai-pmh"},"display":{"title":"In Vitro Selection and Characterization of Catalytic DNA in Model Biological Systems","abstract":"This research also details the first attempt to obtain useful information from heterogeneous DNA sequencing data. A sample of the selection population from each of the twelve rounds of in vitro selection was heterogeneously sequenced without any prior separation or purification. The observed concurrence of qualitative changes in the heterogeneous sequencing chromatograms with observed changes in the catalytic activity of the population is evidence of the ability of heterogeneous DNA sequencing to successfully monitor the evolution of DNA populations during in vitro selection. The ability to successfully analyze heterogeneous DNA samples containing multiple DNA sequences using a single sequencing experiment may play an important role in driving the development of new DNA sequencing technologies and shaping the future of genomic research.","abstract_html":"This research also details the first attempt to obtain useful information from heterogeneous DNA sequencing data. A sample of the selection population from each of the twelve rounds of in vitro selection was heterogeneously sequenced without any prior separation or purification. The observed concurrence of qualitative changes in the heterogeneous sequencing chromatograms with observed changes in the catalytic activity of the population is evidence of the ability of heterogeneous DNA sequencing to successfully monitor the evolution of DNA populations during in vitro selection. The ability to successfully analyze heterogeneous DNA samples containing multiple DNA sequences using a single sequencing experiment may play an important role in driving the development of new DNA sequencing technologies and shaping the future of genomic research.","abstract_has_math":false,"creators":["Slimmer, Scott Collins"],"institution":"University of Illinois at Urbana-Champaign","degree_name":"Ph.D.","degree_level":"Dissertation","degree_discipline":"Materials Science and Engineering","degree_department":null,"school":null,"contributors":["Gerard Wong"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2015,"date_issued":"2015-09-25T20:53:19Z","date_published":"2015-09-25T20:53:19Z","updated_at":"2026-07-22T22:26:20Z","subjects":["Biology, Bioinformatics"],"languages":["eng"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["(MiAaPQ)AAI3363093"],"render_values":[{"text":"(MiAaPQ)AAI3363093","href":null,"code":true}]}]},"links":{"outbound_url":"http://hdl.handle.net/2142/82850","outbound_label":"Handle","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Gerard Wong"]},{"key":"dc:creator","label":"Author","values":["Slimmer, Scott Collins"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2015-09-25T20:53:19Z","10000-01-01","2009"]},{"key":"dc:type","label":"Dc Type","values":["text"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Materials Science and Engineering"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Dissertation"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Ph.D."]},{"key":"thesis:institution_name","label":"Thesis Institution Name","values":["University of Illinois at Urbana-Champaign"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Biology, Bioinformatics"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["eng"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://hdl.handle.net/2142/82850","(MiAaPQ)AAI3363093"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["This research also details the first attempt to obtain useful information from heterogeneous DNA sequencing data. A sample of the selection population from each of the twelve rounds of in vitro selection was heterogeneously sequenced without any prior separation or purification. The observed concurrence of qualitative changes in the heterogeneous sequencing chromatograms with observed changes in the catalytic activity of the population is evidence of the ability of heterogeneous DNA sequencing to successfully monitor the evolution of DNA populations during in vitro selection. The ability to successfully analyze heterogeneous DNA samples containing multiple DNA sequences using a single sequencing experiment may play an important role in driving the development of new DNA sequencing technologies and shaping the future of genomic research.","Made available in DSpace on 2015-09-25T20:53:19Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3363093.pdf: 2426460 bytes, checksum: ecf79e84c4f215a7a5de1c40179d889f (MD5) Previous issue date: 2009","Embargo set by: Seth Robbins for item 84131 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","121 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009."]},{"key":"dc:title","label":"Title","values":["In Vitro Selection and Characterization of Catalytic DNA in Model Biological Systems"]}]}],"canonical_facts":{"dc:contributor":["Gerard Wong"],"dc:creator":["Slimmer, Scott Collins"],"dc:date":["2015-09-25T20:53:19Z","10000-01-01","2009"],"dc:description":["This research also details the first attempt to obtain useful information from heterogeneous DNA sequencing data. A sample of the selection population from each of the twelve rounds of in vitro selection was heterogeneously sequenced without any prior separation or purification. The observed concurrence of qualitative changes in the heterogeneous sequencing chromatograms with observed changes in the catalytic activity of the population is evidence of the ability of heterogeneous DNA sequencing to successfully monitor the evolution of DNA populations during in vitro selection. The ability to successfully analyze heterogeneous DNA samples containing multiple DNA sequences using a single sequencing experiment may play an important role in driving the development of new DNA sequencing technologies and shaping the future of genomic research.","Made available in DSpace on 2015-09-25T20:53:19Z (GMT). No. of bitstreams: 2 license.txt: 4848 bytes, checksum: 96035ab3f5e1c23cc7138a224ce498bd (MD5) 3363093.pdf: 2426460 bytes, checksum: ecf79e84c4f215a7a5de1c40179d889f (MD5) Previous issue date: 2009","Embargo set by: Seth Robbins for item 84131 Lift date: Forever Reason: Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","Restricted to the U of I community idenfinitely during batch ingest of legacy ETDs","U of I Only","121 p.","Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 2009."],"dc:identifier":["http://hdl.handle.net/2142/82850","(MiAaPQ)AAI3363093"],"dc:language":["eng"],"dc:subject":["Biology, Bioinformatics"],"dc:title":["In Vitro Selection and Characterization of Catalytic DNA in Model Biological Systems"],"dc:type":["text"],"thesis:degree_discipline":["Materials Science and Engineering"],"thesis:degree_level":["Dissertation"],"thesis:degree_name":["Ph.D."],"thesis:institution_name":["University of Illinois at Urbana-Champaign"]},"updated_at":"2026-07-22T22:26:20Z"}