Engineering of Aniline Dioxygenase for Bioremediation and Industrial Applications

Another round of saturation mutagenesis on active site residues with 1-K31 as parent followed by random mutagenesis yielded the mutant 3-R21. Whole cell activity of 3-R21 for AN, 24DMA and 2IPA were 27.7, 9.8, and 12 nmol/min/mg protein respectively. The activities of 3-R21 for AN, 24DMA and 2IPA were improved by 8.9, 98.0, and 2.0-fold respectively over its parent 1-K31. In particular, the activity of the final mutant 3-R21 was improved by 3.5-fold over the WT AtdA enzyme. Overall, mutant 3-R21 had three mutations---V205A, 1248L, and S404C.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Chemical Engineering

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Ang, Ee Lui

Contributors dc:contributor

• Zhao, Huimin
• Jeffrey P. Obbard

• Engineering, Chemical

Language dc:language

eng

Identifier

(MiAaPQ)AAI3301098

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/82405