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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.

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Showing 1 to 20 of 31 for “"random mutagenesis"”.

  1. Genetic analysis of Schizosaccharomyces pombe 7SL RNA

    Liao, XiubeiUniversity of Illinois at Urbana-Champaign2011

    … S. pombe 7SL RNA gene by both site-directed and random mutagenesis.

    uiuc Repository record

  2. Engineering of Aniline Dioxygenase for Bioremediation and Industrial Applications

    Ang, Ee LuiUniversity of Illinois at Urbana-Champaign2015

    Another round of saturation mutagenesis on active site residues with 1-K31 as parent followed by random mutagenesis yielded the mutant 3-R21. Whole cell activity of 3-R21 for AN, 24DMA and 2IPA were 27.7, 9.8, and 12 nmol/min/mg protein respectively. The activities of 3-R21 for AN, 24DMA and 2IPA …

    uiuc Repository record

  3. Imaging Chloride Homeostasis in Neurons

    Arosio, DanieleUniversità degli studi di Trento2017

    … in vivo in multiple neurons. Combining random mutagenesis screening, protein engineering and two-photon-imaging this thesis work led to the discovery of new chloride-sensitive GFP mutants and to the establishment of ratiometric imaging procedures for the quantitative combined imaging of …

    trento Repository record

  4. Characterisation of phenol hydroxylase and its auxiliary proteins, DmpM and DmpK, from pseudomonas spl Strain CF600

    Cadieux, Elisabeth2002

    … of the enzyme was inactive. Site-directed and random mutagenesis were performed on DmpM to identify residues involved in its interaction with the oxygenase. Although mutation of six conserved residues activated phenol hydroxylase in steady-state assays, the double mutant M37T/V77A, resulting …

    concordia Repository record

  5. Characterization of the Genes Involved in Biosynthesis and Transport of Schizokinen, a Siderophore Produced by <em>Rhizobium leguminosarum</em> IARI 917.

    Hammond, David Jack2008

    … involved in its biosynthesis using mini Tn5 random mutagenesis. DNA sequence analysis of a non siderophore producing transconjugant revealed a gene involved in PAC/PAS signal transduction systems that respond to many extracellular cues.</p>

    etsu Repository record

  6. Identification of Residues Critical to Epithelial Sodium Channel Assembly

    Whisenant, Ty E.Texas State University-San Marcos2011

    … chain reaction (EP-PCR) was employed to promote random mutagenesis in the extracellular loop of alpha-ENaC. The levels of growth inhibition were monitored in the yeast strain S1InsE4A and subsequently characterized. The location of the point mutations and the corresponding amino acid transitions …

    texas-state Repository record

  7. CRISPR-based approach enables targeted random nucleotide diversification in mammalian cells

    Aiello, DavideUniversità degli studi di Trento2023

    … that had been difficult to generate via rational mutagenesis. Tools for in vivo directed evolution have been mainly developed for bacterial systems, preventing the evolution of proteins in higher organisms in their cellular context. The only tools available for mammalian cells rely on the AID …

    trento Repository record

  8. Structure, Function and Engineering of Peptide -Mhc Binding Receptors

    Chlewicki, Lukasz KrzysztofUniversity of Illinois at Urbana-Champaign2015

    … can influence peptide specificity. Furthermore, random mutagenesis of the TCR revealed that single-site mutations are sufficient to achieve higher-affinities. One mutation in a residue (Valpha1) outside of the CDRs, also generated a higher affinity, peptide specific TCR. Results with this and …

    uiuc Repository record

  9. Structural Dynamics and Signaling Roles of the Aer PAS and F1 Regions

    Campbell, Asharie J.

    … within the PAS sensor. In this study, I used random mutagenesis to identify additional residues in the PAS domain that are critical for FAD-binding, sensing and signal transduction. I found that Aer mutant proteins did not require FAD binding for maturation and stability. However, lesions that …

    loma-linda Repository record

  10. Naujų Mu transpozonų konstravimas ir jų panaudojimas baltymų struktūros tyrimuose /

    Bagdanavičiūtė, Jolita,Institutional Repository of Vilnius University2010

    … transposon Tn-Km-Mre was evaluated by performing random mutagenesis of eco47IR gene. The other task of this work was to find out those regions of the encoded Eco47I restriction endonuclease which tolerate small insertions. Four such regions were identified. Based on this information five positive …

    vilnius Repository record

  11. DEVELOPMENT OF TRAPPING STYLE CASSETTES FOR NEW GENE TARGETING STRATEGIES

    Simsek, SenemTechnische Universität Dresden

    … trapping strategies were developed to enabled random mutagenesis. Today, the goal is to generate more versatile tools to avoid limitations posed by these earlier mutagenesis strategies. Many public and private research centers have united with the aim of mutating all mouse genes. In order to …

    qucosa-diss

  12. The Invariant Arginine In Motif 2 of ESCHERICHIA COLI Alanyl-tRNA Synthetase : Is Important For Catalysis But Not For Substrate Binding

    Lu, Ying

    … 2 of other class II synthetases. Site-directed random mutagenesis has been employed to generate a set of proteins containing amino acid substitutions in a portion of motif 2 of AlaRS. In this set, only mutations at position 69 caused the enzyme to lose ability to complement growth of an …

    loma-linda Repository record

  13. DESIGNING A PLATFORM FOR DEVELOPING NOVEL GLYCAN-BINDING PROTEINS USING YEAST SURFACE DISPLAY

    Mangipudi, AbhishekCornell University2021

    … cancers, and enriched using a combination of random mutagenesis, magnetic-activated cell sorting, and fluorescence-activated cell sorting. The population of isolated binders was characterized by yeast surface titrations. We were able to demonstrate the concentration of binders to …

    cornell Repository record

  14. Unveiling and exploiting <i>Phytophthora capsici</i> effectors and their host targets, with an emphasis on CRN effector proteins

    Marques Monteiro Amaro, Tiago MiguelUniversity of Dundee2017

    … In this thesis, by performing a PCR based random mutagenesis screen on P. capsici CRN effector PcCRN83_152, we showed that PcCRN83_152 cell death is not required for its virulence function. In addition, we demonstrated that PcCRN83_152 interacts with nuclear proteins from the host plants N. …

    dundee Repository record

  15. Progress toward a cloneable bismuth nanoparticle

    Solomon, Courtney, author, Ackerson, Chris, advisor, Snow, Chris, committee member, Tsunoda, Susan, committee memberColorado State University. Libraries2025

    … towards reducing Bi(GSH)3. I generated a random mutagenesis library of ArsC variants, with the next step focusing on screening or selecting for variants that exhibit improved activity towards Bi(GSH)3.

    colostate Repository record

  16. Identifying Sortase A Variants With Higher Catalytic Effeciency

    Suliman, Muna2022

    … the catalytic activity of sortase A. Using random mutagenesis and an in vivo bacterial-based screen we isolated a variant that showed a 13-fold increase in its catalytic efficiency when compared to wild-type. This sortase mutant will enable more efficient labeling of LPETG-tagged substrates …

    vcu Repository record

  17. Genetic and Biochemical Characterization of a Phoa-Dependent Pathway for Phosphite Oxidation in Escherichia Coli

    Yang, KechaoUniversity of Illinois at Urbana-Champaign2015

    … bacterial alkaline phosphatase (BAP). Transposon mutagenesis studies and biochemical isolation of the phosphite-oxidizing component(s) led to the conclusion that BAP alone is sufficient to complete this oxidation reaction. Further biochemical characterization with purified BAP verified that this …

    uiuc Repository record

  18. Expression of Recombinant Chloroperoxidase

    Zong, QinUniversity of Illinois at Urbana-Champaign2015

    … for the site-directed and sequential random mutagenesis studies.

    uiuc Repository record

  19. Characterizing and engineering antibodies against the epidermal growth factor receptor

    Chao, GingerMassachusetts Institute of Technology2008

    … interaction. In our technique, a library of random mutants of the EGFR antigen is displayed on the surface of yeast, and the library is combinatorially selected for loss of binding to the antibody being mapped. If a mutant shows loss of binding to an antibody, then that residue is a potential …

    mit Repository record

  20. Genetic Analysis of the Quorum Sensing Regulator EsaR

    Koziski, Jessica MarieVirginia Tech2008

    … once the AHL complexes to EsaR. Hence, a random mutagenesis genetic approach to isolate EsaR* variants that are immune to the effects of AHL has been utilized. Error-prone PCR and site-directed mutagenesis were used to generate desired mutants, which were subsequently screened for their …

    vt Repository record

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