Applications of Pump-Probe and Two-Photon Techniques in Time-Resolved Fluorescence Microscopy and Spectroscopy

Two-photon fluorescence microscopy has proven to be an useful technique for imaging biological systems. Good spatial resolution, reduced photobleaching, and efficient rejection of excitation wavelength from emitted fluorescence are the main characteristics of this technique. As part of this work, extension of two-photon microscopy to time-resolved polarization imaging of probe molecules inside STO cells are shown. Depending on the type of probe molecules and the nature of their interaction with cellular constituents, different degrees of rotational correlation times are obtained.

Name thesis:degree_name

Ph.D.

Level thesis:degree_level

Dissertation

Discipline thesis:degree_discipline

Physics

Grantor

University of Illinois at Urbana-Champaign

Year dc:date

2015

Author dc:creator

• Dong, Chen-Yuan

Contributors dc:contributor

• Gratton, Enrico

• Physics, Condensed Matter

Language dc:language

eng

Identifier

(MiAaPQ)AAI9904435

OAI identifier oai:identifier

oai:www.ideals.illinois.edu:2142/80655