Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 44 for “"Two-photon microscopy"”.
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Fast scanning two-photon microscopy
Fast scanning two-photon microscopy coupled with the use light activated ion channels provides the basis for fast imaging and stimulation in the characterization of in vivo neural networks. A two-photon microscope capable of fast scanning using acousto-optic deflectors was designed and implemented. …
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On the fundamental imaging-depth limit in two-photon microscopy
One of the principle advantages of two-photon microscopy over one-photon techniques is that it can provide high-resolution images from very deep within living tissue. While imaging depths of 500 micron in brain tissue have become standard performance, larger depths have been inaccessible mainly due …
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Adaptive wavefront correction in two-photon microscopy using Coherence-Gated Wavefront Sensing
The focus of a two-photon microscope is often degraded by inhomogeneities in the refractive index within biological specimens. In this dissertation it is shown for various specimens, even for living zebrafish, that the resolution and the fluorescence signal of a two-photon microscope can be …
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Development of high-speed two-photon microscopy for biological and medical applications
Two-photon microscopy (TPM) is one of the most powerful microscopic technologies for in-vivo 3D tissue imaging up to a few hundred micrometers. It has been finding important applications in neuronal imaging, tumor physiology study, and optical biopsy. A practical limitation of TPM is its slow …
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Two-Photon Microscopy of E-Combretastatin uptake and activation in live mammalian cells
… with UV/visiblelight. Here a novel form of two-photon phototherapy is proposed, in which the "inactive"fnms(£)-combretastatins are used as anticancer pro-drugs that may be activated (converted tothe cw-isomer) by localized irradiation with light at the tumour site. The use of …
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Applications of Pump-Probe and Two-Photon Techniques in Time-Resolved Fluorescence Microscopy and Spectroscopy
Two-photon fluorescence microscopy has proven to be an useful technique for imaging biological systems. Good spatial resolution, reduced photobleaching, and efficient rejection of excitation wavelength from emitted fluorescence are the main characteristics of this technique. As part of this work, …
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Volumetric reconstruction of tissue structure from two-dimensional microscopy images
… Most current methods for tissue analysis use two dimensional histological images of the tissue samples, restricting the analysis to 2D. Existing approaches do not provide essential three-dimensional information such as cell volume, shape and structural orientation of cells within the tissue. …
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High throughput 3D optical microscopy : from image cytometry to endomicroscopy
Optical microscopy is an imaging technique that allows morphological mapping of intracellular structures with submicron resolution. More importantly, optical microscopy is a technique that can readily provide images with biochemical contrast based on different spectroscopic modalities such as …
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Parallel and flexible fluorescent imaging using two-photon RESOLFT super-resolution microscopy with spatial light modulator control
… Optical Fluorescence Transitions) fluorescent microscopy is one technique which can achieve lateral super-resolution imaging. Two-photon microscopy naturally generate high resolution in the longitudinal direction with less background compared to single photon excitation. We combine these two …
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Modulation of GABAergic transmission in the cerebellar stellate cell network by neurotransmitter spillover and synaptic cross talk
… means were employed to study the stellate cell network at the single synapse level. Sustained excitatory input invading the molecular layer was found to depress the chemical transmission between stellate cells, due to a decrease in the release probability of the inhibitory neurotransmitter …
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Using simulation to estimate probability density functions of bound water molecules with DSI
… obtained by 3D microscopic visualization through two photon microscopy of the same tissue. These results provide method for estimating myofiber diameter through the properties of the diffusion PDF obtained by whole tissue magnetic resonance imaging.
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Complex muscle architecture described with diffusion weighted MRI
… derived muscle architecture to 3D whole tissue two-photon microscopy data, and (2) the ability to capture mechanically relevant tongue muscle architecture data from human in vivo and analysis.
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High throughput 3-D tissue cytometry
… has been developed in our laboratory based on two-photon microscopy (TPM) that is capable of in situ 3-D imaging of tissue up to a volume of several mm3 with subcellular resolution. This high throughput tissue cytometry achieves an imaging rate of 2 mm3/hour. I optimize the performance of this …
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Ultrafast optical pulse manipulation in three dimensional-resolved microscope imaging and microfabrication
… aspects of utilizing ultrafast light pulses in two-photon excitation microscopy. First, optical fibers are routinely used in many optical instruments but their use in two-photon microscopy is very limited. As ultrafast light pulses propagate through conventional fiber optics, light pulses are …
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Multi-scale analysis of cardiac myoarchitecture
… imaging (DSI) and high-speed multislice two-photon microscopy (TPM) was used. DSI is a technique that derives fiber orientation from directionality of proton diffusion, whereas TPM derives cellular alignment from an autocorrelation of 3D resolved images of cells and subcellular structure. …
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Elucidation of chemically-induced transdermal transport processes
… have been examined using novel applications of Two-Photon Microscopy (TPM). For the first time, TPM was used to visualize and quantify the oleic acid-induced three-dimensional spatial distributions of rhodamine B hexyl ester (RBHE), a model hydrophobic fluorescent probe, and of sulforhodamine B …
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Applications of pump-probe and two-photon techniques in time-resolved fluorescence miscroscopy and spectroscopy
… and improved spatial resolution similar in two-photon excitation microscopy. Using this technique, spectroscopic data measuring the lifetime and rotational correlation time of aqueous rhodamine B are presented. Furthermore, timeresolved, microscopic images deomonstrate this technique's …
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Engineering the Optical Properties of Gold Nanostructures for Biomedical Applications
… their in vitro targeting capability using two-photon microscopy. This mode of imaging can be used to quickly screen the interaction between Au nanocages and cells, as well as evaluate the distribution of Au nanocages in tissue for ex vivo and in vivo studies. I also quantified the …
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Investigating mechanisms of hemodynamic control in the brain
… These observations guide cellular level two-photon microscopy of neural and glial cell activity. The precise spatiotemporal characteristics of the neurovascular response elucidated in these in vivo studies are then used to construct and constrain a conceptual framework for the signaling …
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